PMID: 9188850Feb 1, 1997Paper

Multiple transcripts of the murine immunoglobulin epsilon membrane locus are generated by alternative splicing and differential usage of two polyadenylation sites

Molecular Immunology
S AnandO R Burrone

Abstract

The human C epsilon gene produces a number of alternatively spliced heavy chain transcripts of which some encode functional IgE isoforms. We now show that differentially processed epsilon mRNA variants also exist in the mouse and are generated by differential polyadenylation and alternative splicing of primary epsilon chain transcripts. The two poly(A) sites of the mouse membrane transcripts were identified in the present study by RACE-PCR analysis. The first poly(A) site is located 743 nt downstream from the beginning of the second membrane exon (M2) and contains the same non-consensus AGTAAA signal sequence as the single poly(A) site of the human membrane transcripts. The second poly(A) site is located almost 500nt further downstream and is characterized by an AAGAAA hexamer. This poly(A) site contains a (G+T) rich element downstream to the site of cleavage and polyadenylation and is preferentially utilized by the membrane epsilon transcripts. Additional diversity of epsilon transcripts is generated by alternative splicing between the last constant region exon (CH4) and the two membrane exons (M1 and M2). The alternatively spliced transcripts include two variants that skip the first membrane exon and encode epsilon heavy chai...Continue Reading

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Citations

Jul 19, 2000·Genome Research·E BeaudoingD Gautheret
Sep 16, 2000·American Journal of Respiratory and Critical Care Medicine·A KarnowskiM C Lamers
Apr 5, 2007·Journal of Genetics·Eillen TecleDavid H Kass
Nov 10, 2011·Immunogenetics·Pheidias C WuTse Wen Chang
Mar 27, 2010·The EMBO Journal·Nuno Miguel NunesAndré Furger
Jan 20, 2005·Gene·Stefan StammHermona Soreq
Nov 29, 2008·Journal of Virological Methods·Giulia Di LulloAntonio G Siccardi
Dec 10, 2016·ELife·Zhiyong YangChristopher D C Allen
May 13, 2017·International Immunology·Annalisa Schaub, Elke Glasmacher
Nov 29, 2017·Frontiers in Immunology·Brice LaffleurMichel Cogné
Feb 13, 2013·The Journal of Allergy and Clinical Immunology·Janet M DaviesRob C Aalberse
Nov 19, 2021·Molecular Therapy : the Journal of the American Society of Gene Therapy·Qiu PengQianjin Liao

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