Multiple vacuolar sorting determinants exist in soybean 11S globulin.

The Plant Cell
Nobuyuki MaruyamaShigeru Utsumi

Abstract

The sorting determinants of glycinin, a soybean (Glycine max) 11S globulin, which mediates protein targeting to the protein storage vacuole (PSV), were investigated in maturing soybean cotyledons by transient expression assays. A C-terminal stretch of 10 amino acids of A1aB1b, a glycinin group I subunit, was sufficient to direct green fluorescent protein (GFP) to the PSV. This peptide may correspond to a C-terminal vacuolar sorting determinant (ctVSD). Because functional inhibition of this putative ctVSD of A1aB1b did not block PSV sorting of A1aB1b, we used the three-dimensional structure of A1aB1b to identify candidates for a sequence-specific determinant (ssVSD). We found that the sequence downstream of disordered region 4 could direct GFP to the PSV and that Ile-297 is critical for sorting. However, functional inhibition of the ctVSD, combined with the Ile297Gly mutation, did not abolish the vacuolar sorting of A1aB1b, suggesting that A1aB1b has a third sorting determinant in addition to ctVSD and ssVSD. A glycinin group II subunit, A3B4, lacked a ctVSD but contained a VSD reminiscent of an ssVSD and an additional sorting determinant. We also demonstrate, by expression of dominant negative mutants of small GTPases and drug ...Continue Reading

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Citations

Oct 8, 2008·Plant Physiology·Inhwan Hwang
Aug 7, 2012·BMC Research Notes·Mona Abirached-DarmencyCharles Schneider
Mar 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Maite SanmartínEnrique Rojo
May 21, 2011·Bioscience, Biotechnology, and Biochemistry·Nobuyuki MaruyamaShigeru Utsumi
May 6, 2014·Journal of Bioscience and Bioengineering·Nobuyuki MaruyamaTeruhiko Terakawa
Dec 2, 2011·Annual Review of Food Science and Technology·Mary Rose G Tandang-SilvasNobuyuki Maruyama
Mar 11, 2010·Biochimica Et Biophysica Acta·Mary Rose G Tandang-SilvasNobuyuki Maruyama
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Mar 7, 2015·Chemical & Pharmaceutical Bulletin·Yasumaru Hatanaka
Jan 20, 2018·Scientific Reports·Nobuyuki MaruyamaMasao Ishimoto

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