Multiple viral infections and genomic divergence among noroviruses during an outbreak of acute gastroenteritis

Journal of Clinical Microbiology
Yukiko SasakiTomoko Yamamoto

Abstract

An epidemic outbreak of both norovirus (NV) and astrovirus (ASV) occurred on a research ship surveying Tokyo Bay, causing acute gastroenteritis in 26 of its 37 crew members. The presence of viral pathogens in fecal specimens was analyzed, and noroviruses were identified by reverse transcription-PCR in 18 (48.6%) of these specimens. In addition, astroviruses were identified in 14 (37.8%) of the fecal samples from the affected crew members, and multiple viral infections of both NV and ASV were observed in 6 cases. The genogrouping of the NV-positive samples was then examined by dot blot hybridization, and it was determined that all of the isolates were from genogroup II (GII). No bacterial pathogens were subsequently isolated from fecal specimens. Furthermore, a variety of NV strains were identified by sequencing and single-stranded conformational polymorphism (SSCP) analyses of PCR products from the fecal samples. One recombinant NV isolate, Minato/14, was identified as a recombinant NV strain of GII/6 and GII/1. The other NV isolates from this outbreak were classified into three NV genotypes (GII/1 [Minato/10], GII/4 [Minato/33], and GII/5 [Minato/6]). Furthermore, ASVs in positive samples were determined to belong to serotypes...Continue Reading

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Citations

May 27, 2010·BMC Infectious Diseases·Nadja ApeltThomas Löscher
Sep 22, 2015·Journal of Medical Virology·Aksara ThongprachumHiroshi Ushijima
Mar 24, 2012·Current Opinion in Virology·Françoise S Le GuyaderJacques Le Pendu
Feb 2, 2010·Journal of Virological Methods·Cheonghoon Lee, Sang-Jong Kim
Jan 14, 2009·Journal of Virological Methods·Pattara KhamrinHiroshi Ushijima
Jan 30, 2013·Journal of Medical Virology·Jones Anderson Monteiro SiqueiraYvone Benchimol Gabbay
Apr 7, 2009·The Journal of Veterinary Medical Science·Satoru AkaseYoshikazu Adachi
Apr 21, 2016·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·José M CuevasRafael Sanjuán
Mar 25, 2008·Journal of Medical Virology·Shinji FukudaMasato Seno
Aug 7, 2008·Journal of Food Protection·Franco PagottoSabah Bidawid
Nov 21, 2007·The Journal of General Virology·Rowena A BullPeter A White

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