PMID: 8589043Oct 1, 1995Paper

Multiplex ARMS-PCR-RFLP method for high-resolution typing of HLA-DRB1

European Journal of Immunogenetics : Official Journal of the British Society for Histocompatibility and Immunogenetics
S MitsunagaT Juji

Abstract

A reliable method for high-resolution HLA-DRB1 typing using the combination of group-specific amplification and RFLP analysis is described. Group-specific PCR amplification (multiplex ARMS-PCR) was carried out under the same conditions for all groups using seven different primer pairs divided into four groups: (1) DR1 and DR10; (2) DR2, DR7 and DR9; (3) DR3 DR5, DR6 and DR8, and (4) DR4. The subsequent polyacrylamide gel electrophoresis was used to determine the group(s) contained in each sample. DR1, DR2/7, DR3/5/6/8, DR4, DRB1*0901 and DRB1*1001 could be distinguished easily using this system. Computer analysis of the various restriction enzyme cleavage sites was carried out on 105 DRB1 allele sequences. It was shown that all DRB1 alleles, except for five allele pairs and some alleles possessing silent mutations, could be distinguished with commonly available restriction endonucleases. Computer analyses on the discrimination of the heterozygous and homozygous combinations were also carried out. Although some heterozygous combinations could no be distinguished with single digestion, double digestion using two restriction enzymes could distinguish most of such heterozygotes. The results of the typing of 100 Japanese individuals...Continue Reading

References

Dec 1, 1992·Quality of Life Research : an International Journal of Quality of Life Aspects of Treatment, Care and Rehabilitation·C A SchagL Petersen
Nov 1, 1992·Tissue Antigens·S G Marsh, J G Bodmer
Feb 1, 1992·Human Immunology·P SantamariaS S Rich
Feb 1, 1992·Tissue Antigens·G BeinH Kirchner
Aug 1, 1991·Tissue Antigens·I YunisE J Yunis
Aug 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·J M TiercyB Mach
Apr 1, 1991·Human Immunology·B Mach, J M Tiercy
Feb 1, 1991·European Journal of Immunogenetics : Official Journal of the British Society for Histocompatibility and Immunogenetics·H ErlichP S Walsh
Jun 1, 1989·The Journal of Experimental Medicine·Y WatanabeT Juji
Apr 1, 1995·Human Immunology·F E Leon-SA Ariza-Caicedo
Jul 1, 1994·Tissue Antigens·J G BodmerT Sasazuki
Jun 1, 1993·European Journal of Immunogenetics : Official Journal of the British Society for Histocompatibility and Immunogenetics·D JawaheerW Thomson
Sep 1, 1993·Tissue Antigens·D ChiaP A Siauw
Feb 1, 1994·European Journal of Immunogenetics : Official Journal of the British Society for Histocompatibility and Immunogenetics·M BannaiT Juji

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.