Multiplex Detection of Criniviruses Associated with Epidemics of a Yellowing Disease of Tomato in Greece

Plant Disease
C I DovasA D Avgelis

Abstract

Since 1997, a yellowing disease has been observed in greenhouse tomato (Lycopersicon escu-lentum). By 2001, the disease was widespread, including open field tomato crops, and in most cases its incidence was 80 to 90% or even 100%. Epidemics in glasshouses were mainly associated with high populations of the whitefly Trialeurodes vaporariorum and Bemisia tabaci, the major whitefly pests in vegetable crops in Greece. The main leaf symptoms were severe yellowing, rolling, and brittleness. Samples from symptomatic plants were analyzed by polymerase chain reaction (PCR) and shown to be infected with Tomato infectious chlorosis virus (TICV) and Tomato chlorosis virus (ToCV) (family Closteroviridae, genus Crinivirus). TICV was found in 164 of 183 symptomatic samples, while ToCV was less representative (25/183). Sequence comparisons of the amplified 229-bp and 466-bp products revealed 99 and 100% identity with the reported sequences of TICV and ToCV, respectively. A reverse transcription (RT) multiplex PCR assay using a simple sample preparation procedure was developed to allow rapid, specific, and simultaneous detection of both ToCV and TICV sequences in two steps. The method involves a one-tube RT-PCR step in which the combination of ...Continue Reading

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Citations

Jul 4, 2019·Molecular Plant Pathology·Elvira Fiallo-Olivé, Jesús Navas-Castillo
Jan 29, 2020·Bulletin of Entomological Research·Vinicius Henrique BelloRenate Krause-Sakate
Dec 24, 2005·Archives of Virology·G LozanoJ Navas-Castillo
Apr 7, 2010·Molecular Plant-microbe Interactions : MPMI·Inge M HanssenBart P H J Thomma
Jun 1, 2006·Plant Disease·William M Wintermantel, Gail C Wisler
Apr 12, 2019·Pakistan Journal of Biological Sciences : PJBS·Aly Mohamed Mamoun Abdel-SalamRehab A Dawoud
Jun 28, 2019·The Plant Pathology Journal·Mehtap Şahin-ÇevikBayram Çevik
Jan 14, 2022·Plant Disease·Erich Yukio Tempel NakasuAlice Inoue-Nagata

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