Multiplex gene editing in rice with simplified CRISPR-Cpf1 and CRISPR-Cas9 systems

Journal of Integrative Plant Biology
Mugui WangJian-Kang Zhu

Abstract

We developed simplified single transcriptional unit (SSTU) CRISPR systems for multiplex gene editing in rice using FnCpf1, LbCpf1 or Cas9, in which the nuclease and its crRNA array are co-expressed from a single Pol II promoter, without any additional processing machinery. Our SSTU systems are easy to construct and effective in mediating multiplex genome editing.

References

Mar 4, 2015·Proceedings of the National Academy of Sciences of the United States of America·Kabin XieYinong Yang
Mar 21, 2017·Molecular Plant·Mugui WangJian-Kang Zhu
May 20, 2017·The Plant Cell·Tomáš ČermákDaniel F Voytas
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Dec 12, 2017·Journal of Integrative Plant Biology·Zhiming YuYiguo Hong

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Citations

Oct 28, 2018·Plant Biotechnology Journal·Rongfang XuPengcheng Wei
Nov 6, 2018·Journal of Integrative Plant Biology·Jochen KumlehnHolger Puchta
Mar 26, 2019·Plant Biotechnology Journal·Hongge JiaNian Wang
Jul 17, 2019·Nature Plants·Yingxiao ZhangYiping Qi
Aug 21, 2019·Microorganisms·Rahul Mahadev ShelakeJae-Yean Kim
Oct 28, 2019·Horticulture Research·Jiemeng XuZhaobo Lang
Jul 30, 2019·Frontiers in Plant Science·Tanushri KaulPawan Kumar Agrawal
Mar 11, 2020·Planta·Shabir Hussain WaniP B Kavi Kishor
Nov 5, 2019·Transgenic Research·Rishikesh GhogareAmit Dhingra
Aug 31, 2018·Plant Cell Reports·Jianping ZhouYong Zhang
Aug 14, 2020·International Journal of Molecular Sciences·Sunny AhmarKi-Hong Jung
Jan 1, 2021·Journal of Plant Physiology·Niklas CapdevilleHolger Puchta
Dec 29, 2020·Journal of Integrative Plant Biology·Xiangqiang ZhanJose Ramon Botella
Mar 17, 2021·Molecular Plant Pathology·Audrey M V Ah-FongHoward S Judelson
Apr 3, 2021·Transgenic Research·Jéssica CarrijoGiovanni R Vianna
Jun 3, 2021·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Xuehan XiaYulin Cheng
Jun 15, 2021·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Qiupeng LinJin-Long Qiu
Jan 12, 2021·Briefings in Bioinformatics·Hui PengJinyan Li
Aug 20, 2021·Journal of Integrative Plant Biology·Taoli LiuQinlong Zhu
Oct 30, 2021·Frontiers in Genome Editing·Shujie DongQiudeng Que
Oct 21, 2021·Plant Biotechnology Journal·Yuechao WuTao Zhang

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