Multiplex Lateral Flow Assay for Rapid Visual Blood Group Genotyping

Analytical Chemistry
Julien Gomez-MartinezJean-Charles Brès

Abstract

Conventional blood group phenotyping by hemagglutination assays, carried out pretransfusion, is unsuitable in certain clinical situations. Molecular typing offers an alternative method, allowing the deduction of blood group phenotype from genotype. However, current methods require a long turnaround time and are not performed on-site, limiting their application in emergency situations. Here, we report the development of a novel, rapid multiplex molecular method to identify seven alleles in three clinically relevant blood group systems (Kidd, Duffy, and MNS). Our test, using a dry-reagent allele-specific lateral flow biosensor, does not require DNA extraction and allows easy visual determination of blood group genotype. Multiplex linear-after-the-exponential (LATE)-PCR and lateral flow parameters were optimized with a total processing time of 1 h from receiving the blood sample. Our assay had a 100% concordance rate between the deduced and the standard serological phenotype in a sample from 108 blood donors, showing the accuracy of the test. Owing to its simple handling, the assay can be operated by nonskilled health-care professionals. The proposed assay offers the potential for the development of other relevant single nucleotid...Continue Reading

References

May 1, 1991·American Journal of Hematology·C RahuelJ P Cartron
Feb 7, 2001·Journal of Clinical Microbiology·W A Al-Soud, P Rådström
Feb 11, 2004·Proceedings of the National Academy of Sciences of the United States of America·J Aquiles SanchezLawrence J Wangh
Jun 7, 2005·Proceedings of the National Academy of Sciences of the United States of America·Kenneth E PierceLawrence J Wangh
Apr 29, 2008·Nucleic Acids Research·Richard OwczarzyAndrew S Peek
Apr 27, 2010·Analytical Chemistry·Mohidus Samad KhanGil Garnier
Apr 5, 2011·Biosensors & Bioelectronics·AngLim ChuaPattabhiraman Lalitha
Dec 14, 2011·Analytical Chemistry·Mohammad Al-TamimiGil Garnier
Feb 23, 2012·Nature Chemistry·David Yu ZhangPeng Yin
Oct 15, 2013·Translational Research : the Journal of Laboratory and Clinical Medicine·Hanane El KenzFrancis Corazza
Jun 23, 2015·Transfusion·Willy A FlegelGregory A Denomme
Jul 25, 2015·The Lancet. Haematology·Willy A FlegelGregory A Denomme
Dec 1, 2015·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·Alhassane BaMonique Silvy
Dec 18, 2015·Blood Transfusion = Trasfusione Del Sangue·Kirstin FinningNúria Nogués
Dec 29, 2015·Blood Transfusion = Trasfusione Del Sangue·Willy A FlegelGregory A Denomme
Apr 16, 2016·Laboratory Medicine·David S Wilkinson
Apr 30, 2016·Analytica Chimica Acta·Temsiri Songjaroen, Wanida Laiwattanapaisal
Jun 28, 2016·Transfusion Medicine Reviews·Ross M Fasano, Stella T Chou
Jul 29, 2016·The New England Journal of Medicine·Michael F MurphyUNKNOWN BEST Collaborative
Nov 29, 2016·Blood Transfusion = Trasfusione Del Sangue·Mónica LópezDiego Tejedor
Jan 4, 2017·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·A BelsitoC Napoli
Mar 17, 2017·Science Translational Medicine·Hong ZhangYang Luo

❮ Previous
Next ❯

Related Concepts

Related Feeds

Biosensor Development & Applications

Biosensor development and applications describe recent progress in biosensor development and their applications in the field of antibiotics and metabolic engineering. Discover the latest research on biosensor development and applications in this feed.

Related Papers

Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis
Nadila Haryani OsmanAsral Wirda Ahmad Asnawi
The Journal of Molecular Diagnostics : JMD
Sandra ParisJean-Charles Brès
© 2022 Meta ULC. All rights reserved