Multiplex measurement of twelve tumor markers using a GMR multi-biomarker immunoassay biosensor

Biosensors & Bioelectronics
Yang GaoYunhua Gao

Abstract

Tumor markers play an important role in the early diagnosis and therapeutic effect monitoring of tumors. Combined detection of multiple tumor markers is a realistic way of improving the sensitivity and specificity of cancer diagnosis. To achieve this, we studied and designed a giant magneto resistance (GMR) multi-biomarker immunoassay biosensor that can simultaneously detect twelve kinds of tumor markers by integrating a GMR sensor chip, a microfluidic device, a magnetic nano-beads label, and a double antibody sandwich immunoassay method. As a proof of concept, the proposed immunosensor was utilized to detect 12 tumor markers (AFP, CEA, CYFRA21-1, NSE, SCC, PG I, PG II, CA19-9, total PSA, free PSA, free-β-hCG, Tg) and to screen patients with lung cancer, liver cancer, digestive tract cancer, prostatic cancer, etc. The immunosensor showed excellent sensitivity, accuracy, precision and stability. Designed as a POCT device, the immunosensor also allows for portability, able to perform rapid detection wherever necessary. As a multi-analyte assay, it provides significant advantages over single-analyte tests in terms of cost per test, labor and convenience. The system's ability to simultaneously measure the concentration of multiple ...Continue Reading

Citations

Jan 1, 2020·Micromachines·Diqing SuJian-Ping Wang
Dec 10, 2020·Nanomaterials·Plengchart PrommapanSteven Lenhert
Jun 1, 2021·Sustainable Cities and Society·Ferial GhaemiMassimiliano Ferrara
Jun 11, 2021·Small·Lude WangHaibo Zeng
Aug 5, 2021·Analytical and Bioanalytical Chemistry·Kaitlynn R MitchellAdam T Woolley
Nov 27, 2019·Analytical Chemistry·Ling XiaGongke Li
Dec 4, 2021·Signal Transduction and Targeted Therapy·Liping ZhongYongxiang Zhao
May 25, 2021·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Nailong GaoHui You

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