Abstract
Tumor markers play an important role in the early diagnosis and therapeutic effect monitoring of tumors. Combined detection of multiple tumor markers is a realistic way of improving the sensitivity and specificity of cancer diagnosis. To achieve this, we studied and designed a giant magneto resistance (GMR) multi-biomarker immunoassay biosensor that can simultaneously detect twelve kinds of tumor markers by integrating a GMR sensor chip, a microfluidic device, a magnetic nano-beads label, and a double antibody sandwich immunoassay method. As a proof of concept, the proposed immunosensor was utilized to detect 12 tumor markers (AFP, CEA, CYFRA21-1, NSE, SCC, PG I, PG II, CA19-9, total PSA, free PSA, free-β-hCG, Tg) and to screen patients with lung cancer, liver cancer, digestive tract cancer, prostatic cancer, etc. The immunosensor showed excellent sensitivity, accuracy, precision and stability. Designed as a POCT device, the immunosensor also allows for portability, able to perform rapid detection wherever necessary. As a multi-analyte assay, it provides significant advantages over single-analyte tests in terms of cost per test, labor and convenience. The system's ability to simultaneously measure the concentration of multiple ...Continue Reading
Citations
Apr 18, 2019·Nanoscale·Yiting JiangPeng Miao
Jan 1, 2020·Micromachines·Diqing SuJian-Ping Wang
Jun 23, 2020·Lab on a Chip·Minjie ShenYouchun Xu
Mar 19, 2020·Micromachines·Seong-Eun KimMin-Ho Lee
Aug 11, 2020·Investigational New Drugs·Bo ChenZhijie Li
Apr 11, 2020·Scientific Reports·Shreya PrakashUmer Hassan
Oct 7, 2020·The Analyst·Dan LiXifan Mei
Dec 10, 2020·Nanomaterials·Plengchart PrommapanSteven Lenhert
Feb 25, 2021·Analytical and Bioanalytical Chemistry·Fanda MengYunhua Gao
Jun 1, 2021·ACS Omega·Fanda MengYunhua Gao
Jun 1, 2021·Sustainable Cities and Society·Ferial GhaemiMassimiliano Ferrara
Jun 11, 2021·Small·Lude WangHaibo Zeng
Aug 8, 2021·Cells·Lucie DescampsAnne-Laure Deman
Aug 5, 2021·Analytical and Bioanalytical Chemistry·Kaitlynn R MitchellAdam T Woolley
Nov 27, 2019·Analytical Chemistry·Ling XiaGongke Li
Nov 11, 2020·Analytical Chemistry·Chenchen WangGaolin Liang
Nov 23, 2021·ACS Sensors·Youlian WeiHongquan Zhu
Dec 4, 2021·Signal Transduction and Targeted Therapy·Liping ZhongYongxiang Zhao
May 25, 2021·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Nailong GaoHui You