Multiplex PCR assay for immediate identification of the infecting species in patients with mycobacterial disease.

Journal of Clinical Microbiology
L F KoxA H Kolk

Abstract

Rapid identification of infecting mycobacterial species enables appropriate medical care decisions to be made. Our aim was to demonstrate the clinical usefulness of the multiplex PCR assay, a test based on PCR, which permits direct identification of 12 mycobacterial species in clinical specimens. A total of 259 specimens from 177 patients who had clinical symptoms of mycobacterial disease but for whom there were difficulties in diagnosis were tested. Specimens were analyzed within 48 h of receipt of the sample. Mycobacteria were identified in 102 specimens; 66 specimens contained nontuberculous mycobacteria, and 36 specimens contained Mycobacterium tuberculosis complex mycobacteria. The PCR assay identified the mycobacterial species in 43 (97.7%) of 44 microscopy- and culture-positive specimens and in 15 (93.8%) of 16 culture-positive, microscopy-negative specimens. It also permitted species identification in infections caused by more than one mycobacterial species. For 56 (96.5%) of the 58 specimens from patients with infections caused by opportunistic mycobacteria, the organisms were identified with the PCR assay. The test was useful also for the identification of fastidious mycobacteria, e.g., M. genavense, and those that ca...Continue Reading

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Citations

Sep 7, 2000·Transactions of the Royal Society of Tropical Medicine and Hygiene·W R FaberF Portaels
Sep 21, 2004·The British Journal of Dermatology·S A AzizA H J Kolk
May 26, 1999·The Journal of Pathology·D Jones, C D Fletcher
Jun 25, 1999·Scandinavian Journal of Infectious Diseases·M E Hillebrand-HaverkortJ H Ten Veen
Feb 14, 2012·Rapid Communications in Mass Spectrometry : RCM·E CrespoF J M Harren
Oct 13, 2006·Current Opinion in Infectious Diseases·V Blackwell
Nov 24, 1999·Journal of Clinical Microbiology·F T KerkhoffA Rothova
Jun 2, 2000·Journal of Clinical Microbiology·L M Pereira Arias-BoudaA H Kolk
Nov 28, 2002·Journal of Clinical Microbiology·Albert García-QuintanillaMaría T Jiménez de Anta
Jun 10, 1998·Journal of Clinical Microbiology·P R KlatserA H Kolk
Jul 3, 1998·Journal of Clinical Microbiology·D van SoolingenJ D van Embden
Aug 31, 2002·Journal of Clinical Microbiology·Lucio RomanoGiovanni Fadda
May 18, 2005·Clinics in Dermatology·William R Faber

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