PMID: 7580915Apr 1, 1995Paper

Multiplex PCR of bcr-abl fusion transcripts in Philadelphia positive acute lymphoblastic leukemia

PCR Methods and Applications
A LeeJerald P Radich

Abstract

We describe a multiplex PCR assay for the detection of bcr-abl fusion mRNA in Philadelphia chromosome positive acute lymphoblastic leukemia (Ph + ALL). The assay provides a quick method for screening p190 (e1:a2) and p210 (b2:a2 or b3:a2) bcr-abl mRNAs simultaneously. The assay proves to be highly sensitive with detection of as little as one positive bcr-abl-expressing cell in a background of 10(5) negative bcr-abl cells. Bone marrow and peripheral blood specimens from six patients were in total accordance when run by multiplex PCR and by the single primer PCR approach. The multiplex bcr-abl assay may prove to be highly useful for screening newly diagnosed patients with ALL for the bcr-abl fusion transcript and in following the course of disease during therapy.

Citations

Dec 9, 2003·Applied and Environmental Microbiology·Tania GonzálezAldo E González
Aug 2, 2001·Cancer Genetics and Cytogenetics·C C So, K F Wong
Oct 23, 1997·Cancer Genetics and Cytogenetics·J R González GarcíaM L Ramírez-Dueñas
Feb 22, 2005·Cancer Genetics and Cytogenetics·Juan Pablo Meza EspinozaJuan Ramón González García
Dec 23, 2008·Seminars in Hematology·Dario Campana
Mar 24, 1999·Scandinavian Journal of Clinical and Laboratory Investigation·H K NogvaI H Madshus
Mar 21, 2013·Journal of Pediatric Hematology/oncology·Manuel Martinez-MancillaPatricio Gariglio
Nov 16, 2006·Proceedings·Gavin Melmed

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