Multiplex PCR to identify macrolide resistance determinants in Mannheimia haemolytica and Pasteurella multocida.

Antimicrobial Agents and Chemotherapy
Simon RoseStephen Douthwaite

Abstract

The bacterial pathogens Mannheimia haemolytica and Pasteurella multocida are major etiological agents in respiratory tract infections of cattle. Although these infections can generally be successfully treated with veterinary macrolide antibiotics, a few recent isolates have shown resistance to these drugs. Macrolide resistance in members of the family Pasteurellaceae is conferred by combinations of at least three genes: erm(42), which encodes a monomethyltransferase and confers a type I MLS(B) (macrolide, lincosamide, and streptogramin B) phenotype; msr(E), which encodes a macrolide efflux pump; and mph(E), which encodes a macrolide-inactivating phosphotransferase. Here, we describe a multiplex PCR assay that detects the presence of erm(42), msr(E), and mph(E) and differentiates between these genes. In addition, the assay distinguishes P. multocida from M. haemolytica by amplifying distinctive fragments of the 23S rRNA (rrl) genes. One rrl fragment acts as a general indicator of gammaproteobacterial species and confirms whether the PCR assay has functioned as intended on strains that are negative for erm(42), msr(E), and mph(E). The multiplex system has been tested on more than 40 selected isolates of P. multocida and M. haemol...Continue Reading

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Jun 29, 2011·Antimicrobial Agents and Chemotherapy·Benoit DesmolaizeStephen Douthwaite

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Citations

Aug 29, 2012·Antimicrobial Agents and Chemotherapy·Niels Møller AndersenStephen Douthwaite
Mar 17, 2016·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Denise Ann Estarez DayaoConny Turni
Sep 28, 2014·The Journal of Antimicrobial Chemotherapy·Anders S OlsenStephen Douthwaite
Mar 20, 2015·Clinical Microbiology Reviews·Xian-Zhi LiHiroshi Nikaido
Dec 12, 2018·Tropical Animal Health and Production·Ling-Cong KongHong-Xia Ma

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