Multiplex PCR using real time DNA amplification for the rapid detection and quantitation of HTLV I or II

Molecular and Cellular Probes
Michael C Estes, J Sanders Sevall

Abstract

A multiplex 'real-time' polymerase chain reaction (PCR) has been established as a general technique for the quantitation of proviral human T-lymphotrophic virus types 1 and 2 (HTLV-I/II). The technology utilizes fluorescence to measure amplification products from the tax gene of Human T-cell lymphotropic virus type 1 or the 5' long terminal repeat of Human T-cell lymphotropic virus type 2. The quantitative amplification of the standard was linear across four orders of magnitude with nearly identical amplification efficiencies for monoplex or the biplex format from 1.4 copes/assay (60 copies proviral DNA/0.5 micrograms human DNA) to 6000 copies/assay (240000 proviral copies/0.5 micrograms human DNA). The human beta-globin gene was used to normalize for human DNA input to determine the proviral DNA load. Three hundred fifty-six specimens received by Specialty Laboratories for HTLV I/II detection provided identical results in the detection of HTLV I/II proviral DNA. No additional positive specimens were identified with the biplex assay format. The coefficient of variation for the proviral DNA load was less than 30% for HTLV I or II quantitation (n=5). For spiked specimens, two groups of five separate 0.25 ml blood specimens (20 to...Continue Reading

References

Mar 1, 1992·Japanese Journal of Cancer Research : Gann·M MochizukiN Miyata
Feb 25, 1989·Lancet·K NishiokaM Osame
Sep 1, 1989·Annals of Neurology·M YoshidaS Sonoda
May 3, 1986·Lancet·M OsameM Tara
Oct 1, 1996·Genome Research·C A HeidP M Williams
Oct 9, 1999·The Journal of Infectious Diseases·A MannsD Waters
Mar 30, 2000·The New England Journal of Medicine·B PoieszR Hutchinson
May 3, 2000·AIDS Research and Human Retroviruses·W J MileyD Waters
Jun 28, 2001·American Journal of Hematology·B J PoieszR Montagna
Feb 9, 2002·Journal of Clinical Laboratory Analysis·P MarkoulatosM Moncany
Mar 7, 2002·Journal of Virological Methods·Axelle DehéeJean Claude Nicolas
May 11, 2002·The Journal of Antimicrobial Chemotherapy·Annemarie Berger, Wolfgang Preiser

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Citations

Feb 1, 2005·Molecular and Cellular Probes·Wendy J WilsonPaula M Imbro
Oct 21, 2004·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Tzong-Hae LeeEdward L Murphy
Feb 17, 2006·Molecular Aspects of Medicine·F WatzingerT Lion
Feb 26, 2011·Journal of Virological Methods·Emanuela A S CostaAdele Caterino-de-Araujo
Aug 6, 2013·Journal of Virological Methods·Carolina RosadasMarzia Puccioni-Sohler
Feb 20, 2007·Revista Da Sociedade Brasileira De Medicina Tropical·Bruna Pedroso Tamegão-LopesJosé Alexandre Rodrigues de Lemos

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