Abstract
Acute bacterial meningitis causes high morbidity and mortality; the associated clinical symptoms often are insensitive or non-specific; and the pathogenic bacteria are geographically diverse. Clinical diagnosis requires a rapid and accurate methodology. This study aimed to develop a new multiplex polymerase chain reaction (mPCR) assay to detect simultaneously six major bacteria that cause adult bacterial meningitis in Taiwan: Klebsiella pneumoniae, Pseudomonas aeruginosa, Streptococcus pneumoniae, Staphylococcus aureus, Escherichia coli, and Acinetobacter baumannii. Species-specific primers for the six bacteria were developed using reference strains. The specificities of the mPCRs for these bacteria were validated, and the sensitivities were evaluated via serial dilutions. The mPCR assay specifically detected all of the six pathogens, particularly with sensitivities of 12 colony forming units (CFU)/mL, 90 CFU/mL, and 390 CFU/mL for E. coli, S. pneumoniae, and K. pneumoniae, respectively. This mPCR assay is a rapid and specific tool to detect the six major bacterial pathogens that cause acute adult meningitis in Taiwan, particularly sensitive for detecting E. coli, S. pneumoniae, and K. pneumoniae. The assay may facilitate early...Continue Reading
References
Apr 1, 1992·Clinical Microbiology Reviews·L D Gray, D P Fedorko
Jan 7, 1993·The New England Journal of Medicine·M L DurandM N Swartz
Feb 24, 1997·Archives of Internal Medicine·B SigurdardóttirS Gudmundsson
Feb 12, 1998·The New England Journal of Medicine·A SchuchatB A Perkins
Feb 18, 1998·Journal of Clinical Microbiology·M du PlessisK P Klugman
Jun 2, 2000·Journal of Clinical Microbiology·J J LuC C Wan
Dec 5, 2000·Journal of the Neurological Sciences·C H LuH W Chang
Sep 28, 2001·Journal of Clinical Microbiology·J C McAvinK L Lohman
Dec 1, 2001·The Journal of Emergency Medicine·J R MinerM Biros
Jul 26, 2002·Clinical Neurology and Neurosurgery·Cheng-Hsien LuHsueh-Wen Chang
Jan 22, 2003·Journal of Applied Microbiology·J XuM J Crowe
Aug 12, 2003·Journal of Medical Microbiology·Lena ClarkeJ Stuart Elborn
Jan 20, 2004·Journal of Medical Microbiology·Karen SmithStuart C Clarke
Feb 10, 2004·Journal of Clinical Microbiology·Tim SchuurmanAnton A van Zwet
Oct 20, 2004·Pediatrics International : Official Journal of the Japan Pediatric Society·Rina UzukaKatsumi Chiba
Oct 21, 2004·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Allan R TunkelRichard J Whitley
Oct 29, 2004·The New England Journal of Medicine·Diederik van de BeekMarinus Vermeulen
Jul 5, 2007·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·C Welinder-OlssonP Larsson
Jul 25, 2007·Infection·D Lepur, B Barsić
Sep 21, 2007·Journal of Dairy Science·H U GraberW Schaeren
Jan 15, 2008·Infection·W-N ChangC-C Chien
Jan 31, 2008·Practical Neurology·Ewout S SchutDiederik van de Beek
Apr 22, 2008·Memórias do Instituto Oswaldo Cruz·Mari TuyamaDavid E Barroso
Feb 6, 2009·Journal of Clinical Microbiology·Mette Kusk BøvingJens Kjølseth Møller
Mar 14, 2009·The Journal of Dairy Research·Syed Riyaz-Ul-HassanGhulam Nabi Qazi