Multiplex polymerase chain reaction for detection of Dirofilaria immitis (Filariidea: Onchocercidae) and Wuchereria bancrofti (Filarioidea: Dipetalonematidae) in their common vector Aedes polynesiensis (Diptera: Culicidae)

Journal of Medical Entomology
L Nicolas, G A Scoles

Abstract

In French Polynesia, Aedes polynesiensis (Marks) is the vector of the human filarial parasite Wuchereria bancrofti (Cobbold) and dog heartworm, Dirofilaria immitis (Leidy). A multiplex polymerase chain reaction (PCR) assay was designed to screen pools of field-collected Ae. polynesiensis for the presence of both parasites simultaneously using primers specific for each parasite. The sensitivity of detection on purified DNA was 1 and 10 pg, equivalent to 0.1 and 1 L3 larva per pool for W. bancrofti and D. immitis, respectively. Codetection was performed at an hybridization temperature of 58 degrees C to avoid competition between heterologous DNA and primers that was observed at 55 degrees C. In addition, D. immitis was detected by PCR in the blood of infected dogs.

Citations

Jun 12, 2002·Transactions of the Royal Society of Tropical Medicine and Hygiene·Reda M R Ramzy
Aug 11, 2011·PLoS Neglected Tropical Diseases·Eric W ChambersStephen L Dobson
Aug 15, 2014·PLoS Neglected Tropical Diseases·Mark A SchmaedickKimberly Y Won
Feb 1, 2008·PLoS Neglected Tropical Diseases·Corey L BrelsfoardStephen L Dobson
Feb 23, 2013·Journal of Medical Entomology·J Marie, H C Bossin
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