Multiplex QTL editing of grain-related genes improves yield in elite rice varieties

Plant Cell Reports
Jianping ZhouYong Zhang

Abstract

Significant yield increase has been achieved by simultaneous introduction of three trait-related QTLs in three rice varieties with multiplex editing by CRISPR-Cas9. Using traditional breeding approaches to develop new elite rice varieties with high yield and superior quality is challenging. It usually requires introduction of multiple trait-related quantitative trait loci (QTLs) into an elite background through multiple rounds of crossing and selection. CRISPR-Cas9-based multiplex editing of QTLs represents a new breeding strategy that is straightforward and cost effective. To test this approach, we simultaneously targeted three yield-related QTLs for editing in three elite rice varieties, namely J809, L237 and CNXJ. The chosen yield-related QTL genes are OsGS3, OsGW2 and OsGn1a, which have been identified to negatively regulate the grain size, width and weight, and number, respectively. Our approach rapidly generated all seven combinations of single, double and triple mutants for the target genes in elite backgrounds. Detailed analysis of these mutants revealed differential contributions of QTL mutations to yield performance such as grain length, width, number and 1000-grain weight. Overall, the contributions are additive, res...Continue Reading

References

Jun 25, 2005·Science·Motoyuki AshikariMakoto Matsuoka
Feb 3, 2006·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Chuchuan FanQifa Zhang
Sep 12, 2006·The Plant Journal : for Cell and Molecular Biology·Seiichi TokiHiroshi Tanaka
Oct 27, 2010·Proceedings of the National Academy of Sciences of the United States of America·Hailiang MaoQifa Zhang
Mar 8, 2013·Current Opinion in Plant Biology·Mayuko IkedaMakoto Matsuoka
Aug 10, 2013·Nature Biotechnology·Qiwei ShanCaixia Gao
Aug 21, 2013·Cell Research·Zhengyan FengJian-Kang Zhu
Sep 19, 2014·Nature Protocols·Qiwei ShanCaixia Gao
Nov 30, 2014·BMC Plant Biology·Hui-Li XingQi-Jun Chen
Mar 4, 2015·Proceedings of the National Academy of Sciences of the United States of America·Kabin XieYinong Yang
Jan 9, 2016·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Chun WangKejian Wang
Apr 25, 2016·Molecular Plant·Xingliang MaYao-Guang Liu
Jun 19, 2016·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Quanlin LiZheng Yuan
Aug 21, 2016·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Rongfang XuJianbo Yang
Nov 2, 2016·Journal of Integrative Plant Biology·Lan ShenKejian Wang
Nov 30, 2016·Frontiers in Plant Science·Levi LowderYiping Qi
Dec 6, 2016·Nature Biotechnology·Bernd ZetscheFeng Zhang
Mar 21, 2017·Molecular Plant·Mugui WangJian-Kang Zhu
Mar 21, 2017·Nature Plants·Dali ZengQian Qian
Mar 30, 2017·Science China. Life Sciences·Lan ShenKejian Wang
May 20, 2017·The Plant Cell·Tomáš ČermákDaniel F Voytas
Jun 7, 2017·Plant Cell Reports·Ying WangXianggan Li
Dec 30, 2017·Science China. Life Sciences·Xiangbing MengKejian Wang
Mar 24, 2018·Molecular Plant·Shaoya LiLanqin Xia
May 16, 2018·Journal of Integrative Plant Biology·Mugui WangJian-Kang Zhu
May 23, 2018·Proceedings of the National Academy of Sciences of the United States of America·Chunbo MiaoJian-Kang Zhu

❮ Previous
Next ❯

Citations

Mar 4, 2020·PloS One·Elia LacchiniMartin M Kater
Apr 1, 2020·International Journal of Molecular Sciences·Bo WangYong Zhang
Apr 3, 2020·Planta·Krishan KumarSujay Rakshit
Mar 30, 2019·Plant Cell Reports·Günther HahneFabien Nogué
Sep 26, 2020·Nature Reviews. Molecular Cell Biology·Haocheng ZhuCaixia Gao
May 8, 2020·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Banashree SaikiaChannakeshavaiah Chikkaputtaiah
Oct 17, 2019·Frontiers in Plant Science·Qiurong RenYong Zhang
Jan 1, 2020·Computational and Structural Biotechnology Journal·Guanqing LiuTao Zhang
Feb 10, 2021·Plant Cell Reports·Xin HuangChangfu Zhu
Jan 6, 2021·Nature Plants·Qiurong RenYong Zhang
May 1, 2021·International Journal of Molecular Sciences·Qier LiuMaoyun She
May 8, 2021·Biological Reviews of the Cambridge Philosophical Society·Ashish Kumar SrivastavaPenna Suprasanna
May 13, 2021·Plant Cell Reports·Sukumar BiswasJianxin Shi
Jun 8, 2021·Journal of Agricultural and Food Chemistry·Mariana Rocha MaximianoOctávio Luiz Franco
May 28, 2021·Plant Biotechnology Journal·Qiurong RenYong Zhang
Jul 16, 2021·Transgenic Research·Jerlie Mhay MatresInez H Slamet-Loedin
Sep 24, 2021·Plant Biotechnology Journal·Jianping ZhouYong Zhang
Oct 30, 2021·Frontiers in Genome Editing·Simon SretenovicYiping Qi
Oct 21, 2021·Plant Biotechnology Journal·Yuechao WuTao Zhang
Nov 27, 2021·Molecular Biology Reports·Antony Ceasar

❮ Previous
Next ❯

Methods Mentioned

BETA
genotyping
targeted mutation
PCR
transfection
electrophoresis
targeted mutations
transgenic

Related Concepts

Related Feeds

CRISPR Ribonucleases Deactivation

CRISPR-Cas system enables the editing of genes to create or correct mutations. This feed focuses on mechanisms that underlie deactivation of CRISPR ribonucleases. Here is the latest research.

CRISPR (general)

Clustered regularly interspaced short palindromic repeats (CRISPR) are DNA sequences in the genome that are recognized and cleaved by CRISPR-associated proteins (Cas). CRISPR-Cas system enables the editing of genes to create or correct mutations. Discover the latest research on CRISPR here.

CRISPR for Genome Editing

Genome editing technologies enable the editing of genes to create or correct mutations. Clustered regularly interspaced short palindromic repeats (CRISPR) are DNA sequences in the genome that are recognized and cleaved by CRISPR-associated proteins (Cas). Here is the latest research on the use of CRISPR-Cas system in gene editing.