Multiplex surface plasmon resonance biosensing and its transferability towards imaging nanoplasmonics for detection of mycotoxins in barley

The Analyst
Sweccha JoshiMichel W F Nielen

Abstract

A 6-plex competitive inhibition immunoassay for mycotoxins in barley was developed on a prototype portable nanostructured imaging surface plasmon resonance (iSPR) instrument, also referred to as imaging nanoplasmonics. As a benchmark for the prototype nanoplasmonics instrument, first a double 3-plex assay was developed for the detection of deoxynivalenol (DON), zearalenone (ZEA), T-2 toxin (T-2), ochratoxin A (OTA), fumonisin B1 (FB1) and aflatoxin B1 (AFB1) using a well-established benchtop SPR instrument and two biosensor chips. To this end, ovalbumin (OVA) conjugates of mycotoxins were immobilized on the chip via amine coupling. The SPR response was then recorded upon injection of a mixture of antibodies at a fixed concentration and the sample (or matrix-matched standard) over a chip with immobilized mycotoxin-OVA conjugates. The chips were regenerated after each sample using 10 mM HCl and 20 mM NaOH and could be used for at least 60 cycles. The limits of detection in barley (in μg kg(-1)) were determined to be 26 for DON, 6 for ZEA, 0.6 for T-2, 3 for OTA, 2 for FB1 and 0.6 for AFB1. Preliminary in-house validation showed that DON, T-2, ZEA and FB1 can be detected at the European Union regulatory limits, while for OTA and A...Continue Reading

References

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Citations

Nov 3, 2016·Journal of Agricultural and Food Chemistry·Sweccha JoshiMichel W F Nielen
Jul 29, 2017·Chemical Reviews·Zdeněk FarkaPetr Skládal
Apr 27, 2019·Analytical and Bioanalytical Chemistry·Zakir HossainChris M Maragos
Apr 4, 2019·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Philana NolanKatrina Campbell
Nov 12, 2017·Analytical and Bioanalytical Chemistry·Riikka PeltomaaMaría C Moreno-Bondi
Jul 28, 2020·Critical Reviews in Analytical Chemistry·Mustafa Oguzhan CaglayanZafer Üstündağ
Apr 25, 2020·Foods·Sofia AgriopoulouTheodoros Varzakas
Nov 28, 2018·Sensors·Riikka PeltomaaMaría C Moreno-Bondi
Nov 26, 2020·Toxins·Christopher Pöhlmann, Thomas Elßner
Jul 31, 2021·Critical Reviews in Food Science and Nutrition·Nevetha RavindranSunil C K
Aug 7, 2021·Toxins·Krisztina Majer-BaranyiAndrás Székács
Feb 18, 2017·Analytical Chemistry·Sweccha JoshiMichel W F Nielen

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