PMID: 11927010Apr 3, 2002Paper

Multiplexed analysis of post-PCR fluorescence-labeled microsatellite alleles and statistical evaluation of their imbalance in brain tumors

Japanese Journal of Cancer Research : Gann
Koji YoshimotoKenshi Hayashi

Abstract

Detection of the loss of chromosomal regions in cancerous tissues has diagnostic and prognostic relevance, and the development of a reliable and cost-effective technique for this is clinically important. Here we present an efficient technique for quantitative detection of microsatellite alleles, using a post-PCR fluorescence-labeling procedure and multiplexed analysis. We also present a new statistical method for the interpretation of the data that permits reliable and sensitive evaluation of the allelic status of sampled DNA. A high-resolution analysis of allelic imbalance on chromosomes 1p, 10 and 19q in 28 glioma samples of various types using this method revealed that allelic imbalances are more frequent than have been reported, suggesting the diagnostic value of this method in examining the genetic profiles of gliomas.

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Citations

Jun 2, 2011·Brain Tumor Pathology·Masahiro MizoguchiTomio Sasaki
Jun 28, 2012·Brain Tumor Pathology·Masahiro MizoguchiTomio Sasaki
Dec 20, 2007·International Journal of Cancer. Journal International Du Cancer·Yanlei GuanKenshi Hayashi
Sep 25, 2018·Pathology International·Shunya TanakaKoji Iihara
Jul 29, 2017·Scientific Reports·Igor P SmirnovEdward N Timofeev
Apr 26, 2006·Journal of Neuro-oncology·Nobuhiro HataTomio Sasaki
Jul 6, 2010·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Yanlei GuanTomio Sasaki
Jul 17, 2021·Brain Tumor Pathology·Masahiro MizoguchiToru Iwaki

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