Multiplexed aptasensor based on metal ions labels for simultaneous detection of multiple antibiotic residues in milk

Biosensors & Bioelectronics
Falan LiXia Sun

Abstract

A dual-target electrochemical aptasensor was developed for the simultaneous detection of multiple antibiotics based on metal ions as signal tracers and nanocomposites as signal amplification strategy. Metal ions such as Cd2+ and Pb2+ could generate distinct differential pulse voltammetry (DPV) peaks. When targets were present, kanamycin (KAN) and streptomycin (STR) as models, the KAN aptamer (KAP) and STR aptamer (STP) were released from their complementary strands, with more change of Cd2+ and Pb2+ corresponding to peak currents. At the same time, complementary strand of KAP (cKAP) and STP (cSTP) were linked with the poly (A) structure (cSTP-PolyA-cKAP) to increase their conformational freedom. Graphitized multi-walled carbon nanotubes (MWCNTGr) and carbon nanofibers-gold nanoparticles (CNFs-AuNPs) as a biosensor platform enhanced the surface area to capture a large amount of cSTP-PolyA-cKAP, thus amplifying the detection response. Under the optimal conditions, the aptasensor could detect KAN and STR as low as 74.50 pM and 36.45 pM respectively with the range from 0.1 to 100 nM and exhibited excellent selectively. Moreover, this aptasensor showed promising applications for the detection of other analytes by changing correspond...Continue Reading

Citations

Apr 20, 2019·Sensors·Xiaojie ShiYemin Guo
Aug 14, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Min JiaXin Bing
Oct 31, 2020·The Science of the Total Environment·Fengling YueXia Sun
Feb 10, 2019·Biosensors & Bioelectronics·Mei LiuNongyue He
May 15, 2021·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Mohamed A FaragHania A Guirguis
Mar 20, 2019·ACS Applied Materials & Interfaces·Xiaoyan HeXiaoquan Lu
Sep 11, 2020·Langmuir : the ACS Journal of Surfaces and Colloids·Jiaojiao ZhouJuewen Liu

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