Multiplexed fluorescence detection of phosphorylation, glycosylation, and total protein in the proteomic analysis of breast cancer refractoriness

Proteomics
Yue GeBrian J Agnew

Abstract

The Multiplexed Proteomics (MP) technology is a new approach that permits quantitative, multicolor fluorescence detection of proteins in one-dimensional or two-dimensional gels. This methodology allows for multiplexed identification and differential analysis of phosphoproteins, glycoproteins, and total proteins within a single gel electrophoresis experiment. Here the MP system was applied to the differential proteomic analysis of pregnancy-induced refractoriness to breast cancer using a rat model system. Differential analyses identified multiple proteins with altered phosphorylation, glycosylation, or protein expression patterns.

References

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Citations

Jan 16, 2007·Journal of Cancer Research and Clinical Oncology·Hai-Jun ZhouPeng-Yuan Yang
Aug 24, 2010·Molecular Biotechnology·Jana CumovaRoman Hajek
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Aug 24, 2006·Expert Review of Anticancer Therapy·Mehdi Nassiri, Mehrdad Nadji
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Sep 5, 2008·Journal of Proteome Research·Helen KimStephen Barnes

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