Multiplexed glycoproteomic analysis of glycosylation disorders by sequential yolk immunoglobulins immunoseparation and MALDI-TOF MS

Proteomics
Luisa SturialeDomenico Garozzo

Abstract

This study applied yolk immunoglobulins immunoaffinity separation and MALDI-TOF MS for clinical proteomics of congenital disorders of glycosylation (CDG) and secondary glycosylation disorders [galactosemia and hereditary fructose intolerance (HFI)]. Serum transferrin (Tf) and alpha1-antitrypsin (AAT) that are markers for CDG, were purified sequentially to obtain high-quality MALDI mass spectra to differentiate single glycoforms of the native intact glycoproteins. The procedure was found feasible for the investigation of protein macroheterogeneity due to glycosylation site underoccupancy then ensuing the characterization of patients with CDG group I (N-glycan assembly disorders). Following PNGase F digestion of the purified glycoprotein, the characterization of protein microheterogeneity by N-glycan MS analysis was performed in a patient with CDG group II (processing disorders). CDG-Ia patients showed a typical profile of underglycosylation where the fully glycosylated glycoforms are always the most abundant present in plasma with lesser amounts of partially and unglycosylated glycoforms in this order. Galactosemia and HFI are potentially fatal diseases, which benefit from early diagnosis and prompt therapeutic intervention. In ...Continue Reading

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Citations

Sep 5, 2009·Analytical and Bioanalytical Chemistry·Lijuang ZhangPengyuan Yang
Feb 18, 2010·Journal of Inherited Metabolic Disease·Nadia Bouteldja, David J Timson
Mar 9, 2011·Journal of Inherited Metabolic Disease·Luisa SturialeDomenico Garozzo
Mar 2, 2011·International Urology and Nephrology·Michael Ludwig, Sidharth K Sethi
Aug 26, 2010·Molecular & Cellular Proteomics : MCP·Sheng PanTeresa A Brentnall
Dec 3, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yannan QinZheng Li
Jul 1, 2009·Expert Opinion on Medical Diagnostics·Jonas Denecke
Dec 23, 2008·Clinica Chimica Acta; International Journal of Clinical Chemistry·Helen MichelakakisEvangelia Dimitriou
Oct 11, 2008·Mass Spectrometry Reviews·Rita BaroneDomenico Garozzo
Oct 31, 2012·Annals of Neurology·Rita BaroneDirk J Lefeber
Aug 27, 2015·Translational Research : the Journal of Laboratory and Clinical Medicine·Monique van ScherpenzeelDirk J Lefeber
May 2, 2015·Electrophoresis·Nicole EngelGünter Allmaier
Jul 21, 2012·Journal of Proteomics·Rita BaroneDomenico Garozzo
Dec 9, 2008·Biochimica Et Biophysica Acta·Harry Schachter, Hudson H Freeze
Jun 21, 2012·Clinica Chimica Acta; International Journal of Clinical Chemistry·Marina MoraitouAgnieszka Pollak
May 4, 2016·Analytical and Bioanalytical Chemistry·Alessandro QuarantaGunnar Thorsén
Sep 21, 2016·Journal of the American Society for Mass Spectrometry·Nicole Y EngelGünter Allmaier
Jul 28, 2016·Journal of Pharmaceutical and Biomedical Analysis·Sachio YamamotoShigeo Suzuki
Sep 19, 2019·Glycoconjugate Journal·Luisa SturialeRita Barone
Dec 8, 2015·Glycoconjugate Journal·Lucia F Zacchi, Benjamin L Schulz
Feb 15, 2021·Allergy·Angela MessinaAndrea Leonardi
Mar 18, 2021·JIMD Reports·Matthew P WilsonJaak Jaeken
Apr 18, 2009·Analytical Chemistry·Mohamed Abu-FarhaDaniel Figeys
Mar 2, 2021·Glycoconjugate Journal·Gabriela Magali PapazogluCarla Gabriela Asteggiano

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