Multiplexed interfacial transduction of nucleic acid hybridization using a single color of immobilized quantum dot donor and two acceptors in fluorescence resonance energy transfer

Analytical Chemistry
W Russ Algar, Ulrich Krull

Abstract

A multiplexed solid-phase assay for the detection of nucleic acid hybridization was developed on the basis of a single color of immobilized CdSe/ZnS quantum dot (QD) as a donor in fluorescence resonance energy transfer (FRET). This work demonstrated that two channels of detection did not necessitate two different QD donors. Two probe oligonucleotides were coimmobilized on optical fibers modified with QDs, and a sandwich assay was used to associate the acceptor dyes with interfacial hybridization events without target labeling. FRET-sensitized acceptor emission provided an analytical signal that was concentration dependent down to 10 nM. Changes in the ratio of coimmobilized probe oligonucleotides were found to yield linear changes in the relative amounts of acceptor emission. These changes were compared to previous studies that used mixed films of two QD donors for two detection channels. The analysis indicated that probe dilution effects were primarily driven by changes in acceptor number density and that QD dilution effects or changes in mean donor-acceptor distance were secondary. Hybridization kinetics were found to be consistent between different ratios of coimmobilized probes, suggesting that hybridization in this type of...Continue Reading

References

Dec 1, 1995·Biophysical Journal·V ChanS E McKenzie
May 22, 2003·Analytical Biochemistry·David EricksonUlrich J Krull
Aug 28, 2003·Nature Materials·Igor L MedintzJ Matthew Mauro
Jun 2, 2005·Nature Materials·Igor L MedintzHedi Mattoussi
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Dec 28, 2005·Nature Materials·Chun-Yang ZhangTza-Huei Wang
Dec 22, 2007·Chemical Reviews·Audrey SassolasLoïc J Blum
Apr 17, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·W Russ Algar, Ulrich J Krull
Jun 20, 2008·Analytical and Bioanalytical Chemistry·Kyle A CissellSapna K Deo
Sep 24, 2008·Angewandte Chemie·Ron GillItamar Willner
Dec 17, 2008·Physical Chemistry Chemical Physics : PCCP·Igor L Medintz, Hedi Mattoussi
Jan 1, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·W Russ Algar, Ulrich J Krull
Feb 27, 2009·Journal of the American Chemical Society·Kelly BoenemanIgor L Medintz
Mar 21, 2009·Analytical Chemistry·Chun-Yang Zhang, Lawrence W Johnson

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Citations

Jun 1, 2010·Analytical and Bioanalytical Chemistry·W Russ Algar, Ulrich J Krull
Jun 10, 2010·Analytical and Bioanalytical Chemistry·Megan K WagnerX Chris Le
Jul 21, 2015·Biosensors & Bioelectronics·Maja StanisavljevicVojtech Adam
Jan 10, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Eleonora PetryayevaUlrich J Krull
Oct 23, 2016·Analytica Chimica Acta·Elena Benito-PeñaMaria C Moreno-Bondi
Sep 7, 2017·Journal of Materials Chemistry. B, Materials for Biology and Medicine·I V MartynenkoY K Gun'ko
Feb 4, 2010·Analytical Chemistry·Chun-yang Zhang, Juan Hu
Aug 2, 2017·Langmuir : the ACS Journal of Surfaces and Colloids·Yi HanUlrich J Krull

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