Multiplexed protein profiling on microarrays by rolling-circle amplification

Nature Biotechnology
Barry SchweitzerStephen F Kingsmore

Abstract

Fluorescent-sandwich immunoassays on microarrays hold appeal for proteomics studies, because equipment and antibodies are readily available, and assays are simple, scalable, and reproducible. The achievement of adequate sensitivity and specificity, however, requires a general method of immunoassay amplification. We describe coupling of isothermal rolling-circle amplification (RCA) to universal antibodies for this purpose. A total of 75 cytokines were measured simultaneously on glass arrays with signal amplification by RCA with high specificity, femtomolar sensitivity, 3 log quantitative range, and economy of sample consumption. A 51-feature RCA cytokine glass array was used to measure secretion from human dendritic cells (DCs) induced by lipopolysaccharide (LPS) or tumor necrosis factor-alpha (TNF-alpha). As expected, LPS induced rapid secretion of inflammatory cytokines such as macrophage inflammatory protein (MIP)-1beta, interleukin (IL)-8, and interferon-inducible protein (IP)-10. We found that eotaxin-2 and I-309 were induced by LPS; in addition, macrophage-derived chemokine (MDC), thymus and activation-regulated chemokine (TARC), soluble interleukin 6 receptor (sIL-6R), and soluble tumor necrosis factor receptor I (sTNF-RI...Continue Reading

References

Nov 1, 1995·Intensive Care Medicine·L G Thijs, C E Hack
Jun 6, 1997·The Journal of Biological Chemistry·M BabaO Yoshie
Jan 27, 1998·The Journal of Biological Chemistry·T ImaiP W Gray
Feb 18, 1999·Molecular and Cellular Biology·S P GygiR Aebersold
May 18, 1999·Analytical Biochemistry·A LuekingG Walter
Jun 8, 1999·European Journal of Immunology·F SallustoA Lanzavecchia
Mar 22, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·E GattiI Mellman
Apr 5, 2000·Nature Biotechnology·A Q Emili, G Cagney
Aug 24, 2000·Proceedings of the National Academy of Sciences of the United States of America·B SchweitzerD C Ward
Sep 6, 2000·Nature Biotechnology·R M de WildtI M Tomlinson
Mar 23, 2001·Nature Immunology·M Moser, K M Murphy
Oct 12, 2000·Current Opinion in Biotechnology·L J HoltI M Tomlinson
Dec 9, 2000·The Journal of Biological Chemistry·P LoetscherI Clark-Lewis
Dec 21, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·J W LillardJ R McGhee
Jan 6, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·S SebastianiA Cavani
Mar 29, 2001·Nature Immunology·B Moser, P Loetscher
Feb 24, 2001·Bratislavské lekárske listy·M Buc, M Bucova
Jun 30, 2001·Trends in Pharmacological Sciences·S Naaby-HansenR Cramer
Jul 14, 2001·Science·B PulendranJ Banchereau
Jul 24, 2001·BioTechniques·M D MoodyC Burns
Aug 8, 2001·International Journal of Experimental Pathology·J R Foster
Dec 1, 2001·Nucleic Acids Research·G NallurB Schweitzer
Jan 16, 2003·Immunology Letters·Bernhard Moser, Lisa Ebert

❮ Previous
Next ❯

Citations

May 28, 2008·Analytical and Bioanalytical Chemistry·Michael Seidel, Reinhard Niessner
Sep 2, 2008·Analytical and Bioanalytical Chemistry·Michael G Roper, Christelle Guillo
Sep 23, 2008·Analytical and Bioanalytical Chemistry·Michael HartmannThomas O Joos
Jul 25, 2012·Analytical and Bioanalytical Chemistry·Kris P F JanssenJeroen Lammertyn
Dec 13, 2006·Biotechnology Letters·Neil K RenaultMarcos J C Alcocer
Dec 1, 2010·Pharmaceutical Research·Luis BerradeJulio A Camarero
Apr 2, 2010·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Marta Sanchez-Carbayo
Apr 30, 2004·Clinica Chimica Acta; International Journal of Clinical Chemistry·Petra PavlickovaHubert Hug
May 13, 2004·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Henry E Neuman de Vegvar, William H Robinson
Dec 10, 2003·Experimental Hematology·Richard D UnwinAnthony D Whetton
Mar 23, 2005·Trends in Biotechnology·Christof M NiemeyerRon Wacker
Aug 15, 2002·Trends in Biotechnology·Christof M Niemeyer
May 14, 2003·Experimental Gerontology·Christian Schöneich
Feb 5, 2003·Current Opinion in Biotechnology·Mats GullbergUlf Landegren
Feb 5, 2003·Current Opinion in Biotechnology·Stephen F Kingsmore, Dhavalkumar D Patel
May 24, 2003·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·J P A BaakE A M Janssen
Jan 28, 2003·Drug Discovery Today·Sean P LalCristobal G dos Remedios
Feb 15, 2005·Drug Discovery Today·Steven BodovitzJutta Bachmann
Apr 6, 2005·Drug Discovery Today·Philipp Angenendt
Feb 7, 2003·Trends in Biotechnology·Young-Sam Lee, Milan Mrksich
Sep 27, 2002·Current Opinion in Pharmacology·Hengli TangFlossie Wong-Staal
Dec 15, 2004·Drug Discovery Today·Michael J Taussig, Ulf Landegren
Oct 21, 2005·The Journal of Molecular Diagnostics : JMD·Gallya GannotMichael R Emmert-Buck
Nov 30, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Jan HesseGerhard J Schütz
Dec 15, 2010·Analytical Chemistry·David C AppleyardPatrick S Doyle
Jan 22, 2011·Analytical Chemistry·Cheng-Sheng HuangBrian T Cunningham
Dec 14, 2011·Analytical Chemistry·Cheng-Sheng HuangBrian T Cunningham
Dec 2, 2009·Analytical Chemistry·Joonhyung LeeCagri A Savran
Feb 13, 2010·Analytical Chemistry·Marcio G von MuhlenScott R Manalis
Apr 2, 2010·ACS Applied Materials & Interfaces·Aaron M LoweNicholas L Abbott
Nov 4, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Yifan WangBin Liu
Jun 3, 2005·Nature·C Garrison FathmanPaul J Utz
Nov 11, 2008·Nature Biotechnology·Sanjeeva Srivastava, Joshua LaBaer
Mar 15, 2006·Nature Medicine·Hongtao ZhangMark I Greene
Jun 5, 2007·Nature Protocols·Jwa-Min NamJay T Groves
Apr 4, 2006·Nature Reviews. Drug Discovery·Stephen F Kingsmore
Jun 3, 2005·Nature Reviews. Genetics·Sascha SauerHans Lehrach
Jul 7, 2005·Nature Reviews. Immunology·Peter FriedlMatthias Gunzer
Jul 23, 2008·The Analyst·Hye Jin LeeRobert M Corn

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.