Multiplexed protein quantitation in Saccharomyces cerevisiae using amine-reactive isobaric tagging reagents.

Molecular & Cellular Proteomics : MCP
Philip L RossDarryl J Pappin

Abstract

We describe here a multiplexed protein quantitation strategy that provides relative and absolute measurements of proteins in complex mixtures. At the core of this methodology is a multiplexed set of isobaric reagents that yield amine-derivatized peptides. The derivatized peptides are indistinguishable in MS, but exhibit intense low-mass MS/MS signature ions that support quantitation. In this study, we have examined the global protein expression of a wild-type yeast strain and the isogenic upf1Delta and xrn1Delta mutant strains that are defective in the nonsense-mediated mRNA decay and the general 5' to 3' decay pathways, respectively. We also demonstrate the use of 4-fold multiplexing to enable relative protein measurements simultaneously with determination of absolute levels of a target protein using synthetic isobaric peptide standards. We find that inactivation of Upf1p and Xrn1p causes common as well as unique effects on protein expression.

References

Feb 18, 1999·Molecular and Cellular Biology·S P GygiR Aebersold
Feb 20, 1999·Cell·M W Hentze, A E Kulozik
Aug 14, 1999·The Journal of Biological Chemistry·F Karimi-BusheriM Weinfeld
Jun 10, 2000·Molecular and Cellular Biology·A B MaderazoA Jacobson
Oct 25, 2001·Gene·C I GonzálezS W Peltz
Jan 29, 2002·Journal of Mass Spectrometry : JMS·Suzana MartinovićRichard D Smith
Jun 18, 2002·Current Opinion in Cell Biology·Alessandro Fatica, David Tollervey
Jul 4, 2002·Molecular & Cellular Proteomics : MCP·Timothy J GriffinRuedi Aebersold
Feb 5, 2003·Current Opinion in Biotechnology·Michael B Goshe, Richard D Smith
Feb 5, 2003·Current Opinion in Biotechnology·W Andy Tao, Ruedi Aebersold
May 29, 2003·Proceedings of the National Academy of Sciences of the United States of America·Scott A GerberSteven P Gygi
Jan 24, 2004·The Journal of Biological Chemistry·Nieves IbarrolaAkhilesh Pandey
Mar 30, 2004·Molecular & Cellular Proteomics : MCP·Kenneth C ParkerStephen Martin

❮ Previous
Next ❯

Citations

Feb 4, 2010·Inflammatory Bowel Diseases·Giulia RodaAldo Roda
Oct 19, 2010·Archives of Toxicology·Mamas MamasRoyston Goodacre
Aug 26, 2006·Analytical and Bioanalytical Chemistry·Peter SchubertJuergen Kast
Oct 11, 2008·Analytical and Bioanalytical Chemistry·Elmar LangenfeldKatrin Marcus
Feb 16, 2010·Analytical and Bioanalytical Chemistry·Julien RoeserHjalmar P Permentier
Jul 1, 2011·Analytical and Bioanalytical Chemistry·Gunnar SchwarzMichael W Linscheid
Jul 15, 2011·Analytical and Bioanalytical Chemistry·Jeroen KoolWilfried M A Niessen
Dec 14, 2011·Analytical and Bioanalytical Chemistry·Andreas P DahlinMagnus Wetterhall
Feb 24, 2012·Analytical and Bioanalytical Chemistry·Amelie S Benk, Christoph Roesli
Mar 14, 2012·Analytical and Bioanalytical Chemistry·Ahmed H El-KhatibMichael W Linscheid
Mar 28, 2012·Analytical and Bioanalytical Chemistry·Caroline EvansPhillip C Wright
Apr 25, 2012·Analytical and Bioanalytical Chemistry·Shao-En Ong
Apr 27, 2012·Analytical and Bioanalytical Chemistry·Anna KonopkaWolf D Lehmann
May 15, 2012·Analytical and Bioanalytical Chemistry·Andy L Christoforou, Kathryn S Lilley
May 23, 2012·Analytical and Bioanalytical Chemistry·Cordula KlockenbuschJuergen Kast
May 31, 2012·Analytical and Bioanalytical Chemistry·Duangnapa KovanichAlbert J R Heck
Jul 20, 2012·Analytical and Bioanalytical Chemistry·Scott J GeromanosJames I Langridge
Jul 10, 2012·Analytical and Bioanalytical Chemistry·Marcus BantscheffBernhard Kuster
Feb 26, 2011·Applied Microbiology and Biotechnology·Bianca PorteusJohn P Bowman
Aug 28, 2007·World Journal of Urology·Rosamonde E BanksPeter J Selby
Mar 5, 2009·Acta Neuropathologica·Maria ZellnerEllen Umlauf
Apr 20, 2007·Molecular Genetics and Genomics : MGG·Jennifer L FuentesJanine R Maddock
Jun 15, 2006·Amino Acids·H SchmidingerR Birner-Gruenberger
Jul 14, 2010·Amino Acids·Bjoern MeyerMichael Karas
Jul 24, 2012·Amino Acids·Ping-Chung ChenJunmin Peng
Dec 18, 2009·Molecules and Cells·Younghee AhnCheolju Lee
Apr 25, 2012·Journal of Inherited Metabolic Disease·Niels GregersenJohan Palmfeldt
Jan 5, 2011·Clinical & Experimental Metastasis·Stuart ThomsonJohn D Haley
Nov 13, 2008·Cytotechnology·Katie F WlaschinWei-Shou Hu
Dec 24, 2008·Digestive Diseases and Sciences·Ying LinRobert R Schade
Sep 6, 2011·Journal of Mammary Gland Biology and Neoplasia·Jamie L Boehmer
Jan 22, 2010·The Protein Journal·Giuseppina MaccarroneDaniel Martins-de-Souza
Aug 2, 2006·Current Psychiatry Reports·Joachim D K UysWillie M U Daniels
Jul 3, 2013·Applied Biochemistry and Biotechnology·Wei Ning Chen, Kee Yang Tan
Jul 18, 2008·Clinical Proteomics·Raghothama ChaerkadyAkhilesh Pandey
Aug 24, 2010·Molecular Biotechnology·Jana CumovaRoman Hajek

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.