Multiplexed sorting of libraries on libraries: a novel method for empirical protein design by affinity-driven phage enrichment on synthetic peptide arrays

Molecular Diversity
Claus Hultschig, Ronald Frank

Abstract

Chemically synthesized peptide arrays on planar cellulose carriers are proposed as libraries of ligands suitable for the multiplexed simultaneous capture of peptide-specific acceptor proteins from a large randomly mutagenized library of acceptor proteins presented on bacteriophage M13 particles. This experimental set-up can be exploited to rapidly screen for individual new, distinct binding partners from two complementary libraries (two-dimensional screening). The technical feasibility of this empirical protein design approach was demonstrated with calmodulin as an aceptor protein using an array of mastoparan variants for multiplexed phage affinity enrichment.

Citations

Oct 28, 2005·Journal of Molecular Recognition : JMR·Rebecca L Rich, David G Myszka
May 14, 2009·Chembiochem : a European Journal of Chemical Biology·Rudolf Volkmer
Dec 7, 2007·Biotechnology & Genetic Engineering Reviews·Kai HilperRobert E W Hancock
Aug 30, 2005·Gene·Zoltán KonthurStefan Dübel

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