Multiplexing cell-cell communication.

Molecular Systems Biology
John T. Sexton, Jeffrey J. Tabor

Abstract

The engineering of advanced multicellular behaviors, such as the programmed growth of biofilms or tissues, requires cells to communicate multiple aspects of physiological information. Unfortunately, few cell-cell communication systems have been developed for synthetic biology. Here, we engineer a genetically encoded channel selector device that enables a single communication system to transmit two separate intercellular conversations. Our design comprises multiplexer and demultiplexer sub-circuits constructed from a total of 12 CRISPRi-based transcriptional logic gates, an acyl homoserine lactone-based communication module, and three inducible promoters that enable small molecule control over the conversations. Experimentally parameterized mathematical models of the sub-components predict the steady state and dynamical performance of the full system. Multiplexed cell-cell communication has applications in synthetic development, metabolic engineering, and other areas requiring the coordination of multiple pathways among a community of cells.

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Citations

May 23, 2021·Trends in Microbiology·Shengbo WuJianjun Qiao

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Datasets Mentioned

BETA
MG1655
U00096.3

Methods Mentioned

BETA
Flow cytometry
biosensor
electrophoresis
PCR
biosensors

Software Mentioned

Bowtie
FlowJo Collectors
DEMUX
BD CellQuest Pro
MEFL
FlowCal
CasValue
AHL
custom Python scripts
FlowCal Python

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