Multiplexing determination of cancer-associated biomarkers by surface-enhanced Raman scattering using ordered gold nanohoneycomb arrays

Bioanalysis
Li LiWenbo Lu

Abstract

Here, a multiplex surface-enhanced Raman scattering (SERS) based assay for simultaneous quantitation of carcinoembryonic antigen (CEA) and α-fetoprotein (AFP) was developed. SERS tags of nanostars and SERS substrates of nanobowl arrays were functionalized with labeling and capturing antibodies, respectively. In presence of antigens, SERS tags, antigens and SERS substrates formed sandwich structure. The SERS-based technique showed a wide linear range from 0.5 to 100 ng/ml and detection limits were 0.41 and 0.35 ng/ml for CEA and AFP in phosphate-buffered saline buffer, respectively. Analysis results of clinical serum samples using this technique were similar to that shown in phosphate-buffered saline buffer. The LODs were 0.44 and 0.40 ng/ml for CEA and AFP, respectively. Conclusion: The precision and stability of this analysis technique were satisfactory, meanwhile, no obvious cross-reactivity could be found. What's more, it also suggested that this novel multiplex SERS-based technique could be a simple, specific, reliable, sensitive and multiplexed tool for important diagnostic and prognostic applications.

References

Jun 16, 2005·Biopolymers·Yasushi NagaseKenji Kamiyama
Mar 15, 2006·BioTechniques·Padma MaruvadaSudhir Srivastava
Jan 6, 2011·Cancer Science·Malin WickströmJoachim Gullbo
Jul 29, 2011·Chemical Society Reviews·Maëlle PerfézouArben Merkoçi
Sep 22, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Yanying RaoWeiping Qian
Oct 27, 2011·Chemical Communications : Chem Comm·Hyangah ChonJaebum Choo
Jul 28, 2012·ACS Nano·Bhaskara V ChikkaveeraiahXiaoyuan Chen
Aug 30, 2012·Clinical Chemistry·Vikram Sheel Kumar, Molly Webster
Mar 6, 2015·Chemical Society Reviews·Li Wu, Xiaogang Qu
Mar 31, 2015·Biosensors & Bioelectronics·Teng XuZhanfang Ma
Jul 15, 2015·Biosensors & Bioelectronics·Jong Uk LeeSang Jun Sim
Nov 3, 2015·Chemical Communications : Chem Comm·Juan LiZhanjun Yang
Apr 28, 2013·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Xiaobo WangJian Shen
Jun 7, 2014·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Na LiuZhanfang Ma
Mar 14, 2016·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Chunyuan SongLianhui Wang

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Methods Mentioned

BETA
surface-enhanced Raman scattering
sandwich immunoassay
infrared spectroscopy
scanning electron microscopy
surface plasmon resonance
amidation
biosensors
biosensor

Software Mentioned

Tensor

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