Multiplicative mononuclear small hepatocytes in adult rat liver: their isolation as a homogeneous population and localization to periportal zone

Biochemical and Biophysical Research Communications
Kinji AsahinaKatsutoshi Yoshizato

Abstract

Adult rat liver contains a minor population of hepatocytes called small hepatocytes (SHs) that are smaller in size and show a higher replicative potential than conventional parenchymal hepatocytes (PHs). However, SHs have been hitherto characterized using a "SH-fraction" that was contaminated with PHs. In the present study, we isolated a PH-free SH-fraction from the adult rat liver using fluorescence-activated cell sorter combined with centrifugal elutriation and characterized the hepatocytes in the fraction. These hepatocytes were designated R3Hs in this study. R3Hs were mononuclear and of lower ploidy. They expressed at high levels genes of Cdc2, connexin 26, hydroxysteroid sulfotransferase, pancreatic secretory trypsin inhibitor, and prostaglandin E2 receptor EP3 subtype. We conclude that SHs dominate the periportal zone in the adult liver, because mRNA or proteins of these genes were exclusively expressed by periportal hepatocytes.

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Citations

May 29, 2007·Biomedical Microdevices·Masumi YamadaTeruo Okano
Apr 19, 2012·Preparative Biochemistry & Biotechnology·Jirka GrosseDaniela Grimm
Jan 22, 2013·Seminars in Cell & Developmental Biology·Andrew W Duncan
Jul 23, 2011·Clinics and Research in Hepatology and Gastroenterology·G GentricC Desdouets
Dec 22, 2007·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Rie UtohKatsutoshi Yoshizato
Mar 13, 2014·Cellular Microbiology·Laura S AustinStefan H I Kappe
Oct 22, 2013·The American Journal of Pathology·Géraldine Gentric, Chantal Desdouets
Dec 13, 2016·Nature Reviews. Genetics·Lars A ForsbergJan P Dumanski
May 19, 2017·Cell Death & Disease·Min-Jun WangYi-Ping Hu
Apr 4, 2020·Nature Reviews. Gastroenterology & Hepatology·Romain DonneChantal Desdouets
Nov 23, 2017·Frontiers in Physiology·Clemens KreutzMaría M Bartolomé-Rodríguez
Sep 25, 2009·Cell Transplantation·Manabu NishieKatsutoshi Yoshizato

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