PMID: 6894731May 1, 1981Paper

Multiplicity of cytochrome P-450 in primary fetal hepatocytes in culture

European Journal of Biochemistry
P KremersJ E Gielen

Abstract

Primary fetal rat hepatocytes in culture display different monooxygenase activities which can be induced by several chemical inducers. Until now, these hepatocytes were believed to produce only one single cytochrome P-450 species, namely the cytochrome P1-450 (or P-448). It now seems possible to induce other cytochrome P-450 species in these hepatocytes, providing that they receive an appropriate hormonal treatment. We have examined the effect of dexamethasone on the cytochrome P-450 type supporting different enzymic activities (aryl hydrocarbon hydroxylase, ethoxycoumarin deethylase, aldrin monooxygenase). Our results show that the presence of dexamethasone in the culture medium produces qualitative and quantitative changes in the monooxygenase-supporting cytochrome(s) P-450. For low dexamethasone concentrations (between 1 nM and 0.1 microM) a cytochrome P-450 is formed displaying biochemical and biophysical properties similar to those induced by phenobarbital in the adult rat liver. At higher concentrations (above 1 microM), similar qualitative changes are observed; but a quantitative phenomenon occurs, the (cytochrome P-450)-dependent enzymic activities being also induced. Dexamethasone also has a synergistic effect on the i...Continue Reading

References

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Citations

Nov 1, 1993·The International Journal of Biochemistry·J T BorlakogluC R Wolf
Sep 1, 1987·Cell Differentiation·A BollinneJ E Gielen
Dec 1, 1988·Molecular and Cellular Endocrinology·J M MathisR A Prough
May 15, 1983·Biochemical Pharmacology·J M BegueA Guillouzo

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