PMID: 6894731May 1, 1981Paper

Multiplicity of cytochrome P-450 in primary fetal hepatocytes in culture

European Journal of Biochemistry
P KremersJ E Gielen


Primary fetal rat hepatocytes in culture display different monooxygenase activities which can be induced by several chemical inducers. Until now, these hepatocytes were believed to produce only one single cytochrome P-450 species, namely the cytochrome P1-450 (or P-448). It now seems possible to induce other cytochrome P-450 species in these hepatocytes, providing that they receive an appropriate hormonal treatment. We have examined the effect of dexamethasone on the cytochrome P-450 type supporting different enzymic activities (aryl hydrocarbon hydroxylase, ethoxycoumarin deethylase, aldrin monooxygenase). Our results show that the presence of dexamethasone in the culture medium produces qualitative and quantitative changes in the monooxygenase-supporting cytochrome(s) P-450. For low dexamethasone concentrations (between 1 nM and 0.1 microM) a cytochrome P-450 is formed displaying biochemical and biophysical properties similar to those induced by phenobarbital in the adult rat liver. At higher concentrations (above 1 microM), similar qualitative changes are observed; but a quantitative phenomenon occurs, the (cytochrome P-450)-dependent enzymic activities being also induced. Dexamethasone also has a synergistic effect on the i...Continue Reading


Nov 15, 1979·Biochemical Pharmacology·P WiebkinJ W Bridges
Jan 1, 1978·Methods in Enzymology·H W Strobel, J D Dignam
Jan 1, 1978·Annual Review of Pharmacology and Toxicology·G J Dutton
Feb 28, 1979·Biochemical and Biophysical Research Communications·M Lambiotte, J Sjövall
Dec 30, 1977·Archives of Toxicology·V Ullrich, P Kremers
Sep 9, 1977·Biochemical and Biophysical Research Communications·G M DecadJ L Byard
Jan 2, 1975·Biochemical and Biophysical Research Communications·V UllrichP Wollenberg
Jun 16, 1975·European Journal of Biochemistry·J E GielenJ Renson
Jul 15, 1974·European Journal of Biochemistry·P MoldéusS Orrenius
May 15, 1980·Analytical Biochemistry·J De GraeveJ E Gielen
May 30, 1980·Biochemical and Biophysical Research Communications·F J WiebelM Lambiotte
May 1, 1980·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·D J BenfordD V Parke


Nov 1, 1993·The International Journal of Biochemistry·J T BorlakogluC R Wolf
Sep 1, 1987·Cell Differentiation·A BollinneJ E Gielen
Dec 1, 1988·Molecular and Cellular Endocrinology·J M MathisR A Prough
May 15, 1983·Biochemical Pharmacology·J M BegueA Guillouzo

Related Concepts

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Hereditary Sensory Autonomic Neuropathy

Hereditary Sensory Autonomic Neuropathies are a group of inherited neurodegenerative disorders characterized clinically by loss of sensation and autonomic dysfunction. Here is the latest research on these neuropathies.

Spatio-Temporal Regulation of DNA Repair

DNA repair is a complex process regulated by several different classes of enzymes, including ligases, endonucleases, and polymerases. This feed focuses on the spatial and temporal regulation that accompanies DNA damage signaling and repair enzymes and processes.

Glut1 Deficiency

Glut1 deficiency, an autosomal dominant, genetic metabolic disorder associated with a deficiency of GLUT1, the protein that transports glucose across the blood brain barrier, is characterized by mental and motor developmental delays and infantile seizures. Follow the latest research on Glut1 deficiency with this feed.

Separation Anxiety

Separation anxiety is a type of anxiety disorder that involves excessive distress and anxiety with separation. This may include separation from places or people to which they have a strong emotional connection with. It often affects children more than adults. Here is the latest research on separation anxiety.

KIF1A Associated Neurological Disorder

KIF1A associated neurological disorder (KAND) is a rare neurodegenerative condition caused by mutations in the KIF1A gene. KAND may present with a wide range and severity of symptoms including stiff or weak leg muscles, low muscle tone, a lack of muscle coordination and balance, and intellectual disability. Find the latest research on KAND here.

Regulation of Vocal-Motor Plasticity

Dopaminergic projections to the basal ganglia and nucleus accumbens shape the learning and plasticity of motivated behaviors across species including the regulation of vocal-motor plasticity and performance in songbirds. Discover the latest research on the regulation of vocal-motor plasticity here.