Multipotency of melanoblasts isolated from murine skin depends on the Notch signal

Developmental Dynamics : an Official Publication of the American Association of Anatomists
Natsuki WatanabeTakahiro Kunisada

Abstract

Melanoblasts (MBs), derived from neural crest cells, only differentiate into melanocytes (Ms) in vivo. We previously showed that MBs isolated from mouse skin were multipotent, generating neurons (Ns) and glial cells (Gs) together with Ms. Using Sox10-IRES-Venus mice and mouse embryonic stem cells, we investigated how MBs expressed their multipotency. MBs generated colonies containing Ns, Gs, and Ms in the presence of ST2 stromal cells, but they generated only M colonies when incubated with keratinocytes or ST2 culture supernatant, thus showing that MBs required contact with ST2 stromal cells for expression of their multipotency. Notch signaling was shown to be one of the important cues for the maintenance and differentiation of MBs through cell-cell contact. When Notch signaling was inhibited, MBs mainly generated colonies that contained just one type of cells, Ns, Gs, or Ms; the number of colonies containing two or three types of cells markedly decreased even on ST2 stromal cells, showing restriction of their differentiation potency. Whereas when Notch signaling was activated, the number of colonies containing two or three types of cells increased, indicating that their multipotency had been maintained. Our results demonstrate...Continue Reading

References

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Apr 8, 2009·Stem Cells·Tsutomu MotohashiTakahiro Kunisada
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May 20, 2011·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Tsutomu MotohashiTakahiro Kunisada
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Nov 26, 2013·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Tsutomu MotohashiTakahiro Kunisada

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Citations

Mar 28, 2018·Pigment Cell & Melanoma Research·Huirong Li, Ling Hou
Jul 23, 2019·The Journal of Investigative Dermatology·Melissa CrawfordLina Dagnino
Nov 27, 2021·Journal of Periodontal Research·Yuta ShimizuKen-Ichi Tezuka

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