Multipotent Adult Progenitor Cells Support Lymphatic Regeneration at Multiple Anatomical Levels during Wound Healing and Lymphedema

Scientific Reports
Manu BeerensAernout Luttun

Abstract

Lymphatic capillary growth is an integral part of wound healing, yet, the combined effectiveness of stem/progenitor cells on lymphatic and blood vascular regeneration in wounds needs further exploration. Stem/progenitor cell transplantation also emerged as an approach to cure lymphedema, a condition caused by lymphatic system deficiency. While lymphedema treatment requires lymphatic system restoration from the capillary to the collector level, it remains undetermined whether stem/progenitor cells support a complex regenerative response across the entire anatomical spectrum of the system. Here, we demonstrate that, although multipotent adult progenitor cells (MAPCs) showed potential to differentiate down the lymphatic endothelial lineage, they mainly trophically supported lymphatic endothelial cell behaviour in vitro. In vivo, MAPC transplantation supported blood vessel and lymphatic capillary growth in wounds and restored lymph drainage across skin flaps by stimulating capillary and pre-collector vessel regeneration. Finally, human MAPCs mediated survival and functional reconnection of transplanted lymph nodes to the host lymphatic network by improving their (lymph)vascular supply and restoring collector vessels. Thus, MAPC tra...Continue Reading

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Citations

Aug 7, 2019·Journal of Surgical Oncology·Ching-En ChenChih-Hsun Lin
Dec 20, 2018·Journal of Biological Engineering·Laura AlderferDonny Hanjaya-Putra
Aug 28, 2020·World Journal of Stem Cells·Zhu-Jun LiXiao Long
Jan 28, 2021·Advanced Healthcare Materials·Wenkai JiaFeng Zhao
Mar 4, 2021·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Parinaz AhangarAllison J Cowin

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Methods Mentioned

BETA
fluorescence imaging
Fluorescence
circumcision
FACS
biopsy

Software Mentioned

Graphpad Prism
Image Lab
ImageJ

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