Multipotent mesenchymal stromal cells from patients with newly diagnosed type 1 diabetes mellitus exhibit preserved in vitro and in vivo immunomodulatory properties

Stem Cell Research & Therapy
Juliana Navarro Ueda YaochiteKelen Cristina Ribeiro Malmegrim

Abstract

Type 1 diabetes mellitus (T1D) is characterized by autoimmune responses resulting in destruction of insulin-producing pancreatic beta cells. Multipotent mesenchymal stromal cells (MSCs) exhibit immunomodulatory potential, migratory capacity to injured areas and may contribute to tissue regeneration by the secretion of bioactive factors. Therefore, MSCs are considered as a promising approach to treat patients with different autoimmune diseases (AID), including T1D patients. Phenotypical and functional alterations have been reported in MSCs derived from patients with different AID. However, little is known about the properties of MSCs derived from patients with T1D. Since autoimmunity and the diabetic microenvironment may affect the biology of MSCs, it becomes important to investigate whether these cells are suitable for autologous transplantation. Thus, the aim of the present study was to evaluate the in vitro properties and the in vivo therapeutic efficacy of MSCs isolated from bone marrow of newly diagnosed T1D patients (T1D-MSCs) and to compare them with MSCs from healthy individuals (C-MSCs). T1D-MSCs and C-MSCs were isolated and cultured until third passage. Then, morphology, cell diameter, expression of surface markers, di...Continue Reading

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Citations

Mar 2, 2019·Diabetology & Metabolic Syndrome·Mairim Alexandra SolisLynn L H Huang
Sep 19, 2018·International Journal of Molecular Sciences·Arianna Scuteri, Marianna Monfrini
May 29, 2019·Integrative Biology : Quantitative Biosciences From Nano to Macro·Evelyn Kendall WilliamsAndrés J García
Sep 1, 2018·Current Diabetes Reports·Laura M JacobsenTodd M Brusko
Oct 10, 2020·World Journal of Stem Cells·Nazmul HaquePratiwi Soesilawati

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Datasets Mentioned

BETA
E-MTAB-2976

Methods Mentioned

BETA
flow cytometry
light microscopy
FACS
ELISA

Software Mentioned

R
Linear Models for Microarray Data ( LIMMA
CellQuest Pro
GenePattern
HeatMapViewer

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