Multiscale nonlinear microscopy and widefield white light imaging enables rapid histological imaging of surgical specimen margins

Biomedical Optics Express
Michael G GiacomelliJames G Fujimoto

Abstract

The ability to histologically assess surgical specimens in real-time is a long-standing challenge in cancer surgery, including applications such as breast conserving therapy (BCT). Up to 40% of women treated with BCT for breast cancer require a repeat surgery due to postoperative histological findings of close or positive surgical margins using conventional formalin fixed paraffin embedded histology. Imaging technologies such as nonlinear microscopy (NLM), combined with exogenous fluorophores can rapidly provide virtual H&E imaging of surgical specimens without requiring microtome sectioning, facilitating intraoperative assessment of margin status. However, the large volume of typical surgical excisions combined with the need for rapid assessment, make comprehensive cellular resolution margin assessment during surgery challenging. To address this limitation, we developed a multiscale, real-time microscope with variable magnification NLM and real-time, co-registered position display using a widefield white light imaging system. Margin assessment can be performed rapidly under operator guidance to image specific regions of interest located using widefield imaging. Using simulated surgical margins dissected from human breast excis...Continue Reading

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Citations

Nov 21, 2019·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Lucas C CahillJames G Fujimoto
Jul 12, 2019·Archives of Pathology & Laboratory Medicine·Savitri KrishnamurthyMilind Rajadhyaksha
Feb 10, 2019·Journal of Biomedical Optics·Weisi XieJonathan T C Liu
Aug 28, 2019·Biomedical Optics Express·Michael G GiacomelliJames G Fujimoto
Mar 28, 2019·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Lucas C CahillSeymour Rosen
Mar 21, 2019·Biomedical Optics Express·Ye ChenJonathan T C Liu
Jul 8, 2020·Scientific Reports·Evangelia GavgiotakiIrene Athanassakis
Oct 19, 2019·Journal of Biomedical Optics·Michael G Giacomelli
Feb 17, 2021·Nature Biomedical Engineering·Jonathan T C LiuAnant Madabhushi
Mar 6, 2021·Scientific Reports·Vincent D Ching-RoaMichael G Giacomelli
Nov 3, 2021·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Lucas C CahillYue Sun

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