Multispectroscopic and bioimaging approach for the interaction of rhodamine 6G capped gold nanoparticles with bovine serum albumin

Journal of Photochemistry and Photobiology. B, Biology
N ManjubaashiniT Daniel Thangadurai

Abstract

Binding interaction of Bovine Serum Albumin (BSA) with newly prepared rhodamine 6G-capped gold nanoparticles (Rh6G-Au NPs) under physiological conditions (pH 7.2) was investigated by a wide range of photophysical techniques. Rh6G-Au NPs caused the static quenching of the intrinsic fluorescence of BSA that resulted from the formation of ground-state complex between BSA and Rh6G-Au NPs. The binding constant from fluorescence quenching method (Ka = 1.04 × 104 L mol-1; LoD = 14.0 μM) is in accordance with apparent association constant (Kapp = 1.14 × 101 M-1), which is obtained from absorption spectral studies. Förster resonance energy transfer (FRET) efficiency between the tryptophan (Trp) residue of BSA and fluorophore of Rh6G-Au NPs during the interaction was calculated to be 90%. The free energy change (ΔG = -23.07 kJ/mol) of BSA-Rh6G-Au NPs complex was calculated based on modified Stern-Volmer Plot. The time-resolved fluorescence analysis confirmed that quenching of BSA follows static mechanism through the formation of ground state complex. Furthermore, synchronous and three-dimensional fluorescence measurement, Raman spectral analysis and Circular Dichroism spectrum results corroborate the strong binding between Rh6G-Au NPs an...Continue Reading

Citations

Nov 5, 2019·Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes·Yanxiu GuoWansong Zong
Feb 7, 2019·Luminescence : the Journal of Biological and Chemical Luminescence·Lining ZhaoRutao Liu
Apr 30, 2021·Expert Opinion on Therapeutic Patents·José Marcos Teixeira de Alencar FilhoLarissa Araújo Rolim
Jun 18, 2021·Nano-micro Letters·Yuzhen OuyangMinghua Wu
Dec 11, 2021·Journal of Biomolecular Structure & Dynamics·Shunmugasundaram GurusamyAlagarsamy Mathavan

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