Multistage chemical carcinogenesis protocols produce spindle cell carcinomas of the mouse skin.

Carcinogenesis
A J Klein-SzantoC J Conti

Abstract

Spindle cell carcinomas were identified using polyacrylamide gel electrophoresis and immunoblotting of proteins extracted from paraffin-embedded tissue sections. Immunohistochemistry using rabbit monospecific antisera against the mouse 55 kd keratin polypeptide also identified these tumors. A group of 53 SENCAR mice initiated with 7,12-dimethylbenz[a]anthracene (DMBA) and promoted with 12-O-tetradecanoylphorbol-13-acetate (TPA) yielded, after one year, four spindle cell carcinomas (0.07/mouse), whereas another group of 31 mice treated with a three-stage carcinogenesis protocol (initiation with DMBA and promotion for 10 weeks with TPA followed by 10 weeks of benzoyl peroxide) gave rise to six spindle cell carcinomas (0.19/mouse). The number of keratin-positive tumor cells and the intensity of the immunostain varied markedly, but all tumors expressed the 55 kd polypeptide. Although other carcinogens, mainly UV radiation, have been able to induce spindle cell tumors, the present data indicate that chemical carcinogenesis protocols are able to induce the formation of this highly malignant variant of skin carcinoma.

Citations

May 26, 1999·The Journal of Pathology·R J Akhurst, A Balmain
Jun 1, 1995·Cancer Metastasis Reviews·K Brown, A Balmain
Jan 1, 1990·Experimental Pathology·J M Ward, S Rehm
Sep 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·A B BianchiC J Conti
Oct 19, 2007·Cancer Research·Everardo MaciasMarcelo L Rodriguez-Puebla
Jun 4, 2004·Journal of Carcinogenesis·Yan ChenThomas G O'Brien
Apr 5, 2005·Oncogene·Eduardo Pérez-GómezMiguel Quintanilla
Oct 22, 2008·Journal of Dermatological Science·Fernando BenavidesDonna F Kusewitt
Aug 20, 2010·Molecular Carcinogenesis·Stephanie H ShirleyDonna F Kusewitt
Oct 6, 2007·The American Journal of Pathology·Kimberly M NewkirkDonna F Kusewitt
Apr 12, 2014·Seminars in Cancer Biology·Ihn Young Song, Allan Balmain
Sep 8, 1995·Cell·E SaezB M Spiegelman
Oct 27, 1999·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·M DaviesS S Prime
Jul 9, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Ricardo López de CiccoAndrés J Klein-Szanto

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