Multistage magnetic separation of microspheres enabled by temperature-responsive polymers

ACS Applied Materials & Interfaces
Jiamin WuDi Gao

Abstract

A simple and rapid method for separation of cells is to functionalize magnetic particles with a receptor that selectively captures the target and then pull the magnetic particles out of the mixture upon applying a magnetic field. The separation efficiency of magnetic separation, however, is typically limited by the nonspecific interaction between the magnetic particles and nontarget species. We here present a multistage separation process that is able to effectively circumvent the problem caused by the nonspecific interactions by introducing multiple capture-and-release cycles to the magnetic separation process. The multiple capture-and-release cycles are enabled by attaching a temperature-responsive polymer to both the magnetic particles and the targets. Through temperature cycling, we demonstrate that target microspheres can be separated from nontarget microspheres in multiple separation stages. The overall enrichment factor significantly increases with the number of separation stages and reaches as high as 1.87 × 10(5) after 5 cycles.

References

Jan 1, 1990·Cytometry·S MiltenyiA Radbruch
May 12, 1989·Journal of Immunological Methods·G C Douglas, B F King
Mar 1, 1994·Applied Microbiology and Biotechnology·A KondoK Higashitani
Mar 6, 1999·Journal of Chromatography. B, Biomedical Sciences and Applications·I Safarík, M Safaríková
Jul 10, 2002·Biomaterials·Yong HuChangzheng Yang
May 20, 2003·Immunology and Cell Biology·Aruna P KodituwakkuDon M Roberton
Jun 10, 2004·Journal of the American Chemical Society·Jinho HyunStefan Zauscher
Apr 1, 2005·Biotechnology and Bioengineering·Jae-Young KimWilfred Chen
Jan 19, 2006·Journal of the American Chemical Society·Di GaoWilfred Chen
Apr 29, 2006·Analytical Chemistry·Joshua K HerrWeihong Tan
Feb 24, 2009·Proceedings of the National Academy of Sciences of the United States of America·AmirAli H TalasazRonald W Davis
Jul 25, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·M Carmen MoránBjörn Lindman
May 26, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Marta A Cooperstein, Heather E Canavan
Sep 19, 2006·Soft Matter·Robert J MartRein V Ulijn

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Citations

Oct 19, 2013·ACS Applied Materials & Interfaces·Qipeng ZhaoXianmao Lu

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