Multistep microsatellite mutation in the maternally transmitted locus D13S317: a case of maternal allele mismatch in the child

International Journal of Legal Medicine
Devinder Singh NegiJavaregowda Nagaraju

Abstract

Examination of a case of a paternity dispute with 17 autosomal short tandem repeats (STR) loci revealed a mismatch of the maternally transmitted allele at the locus D13S317 in the questioned child. The composition of the alleles of this locus in the mother, questioned child and suspected father was 8/8, 11/11 and 8/11, respectively. The sequence analysis of the regions flanking the locus D13S317 and peak height measurements of the paternal, maternal and child alleles at this locus excluded the possibility of null allele as a cause of the allelic mismatch inherited by the child. The results suggested expansion of the microsatellite repeat motif, TATC by three repeat units as a probable cause for the allelic mismatch in the child. This is a rare case of maternally transmitted multistep microsatellite mutation reported for the first time for this locus in the forensic DNA analysis. The mutation rate at D13S317 locus in maternal and paternal meiosis was 0.04 and 0.14%, respectively, and overall mutation rate was 0.15%. The probability of maternity and paternity were 0.999999 and 0.999999, respectively, for all the 17 autosomal STR loci analyzed. Furthermore, the sequence of two hypervariable regions of mitochondrial DNA, HV1 and HV...Continue Reading

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Citations

Apr 5, 2007·International Journal of Legal Medicine·Nancy VanderheydenRonny Decorte
Nov 12, 2009·International Journal of Legal Medicine·Miriam MüllerBernd Brinkmann
Aug 30, 2011·Forensic Science International. Genetics·Eva-Maria DauberWolfgang R Mayr
Mar 20, 2009·Clinica Chimica Acta; International Journal of Clinical Chemistry·Venkanna NarkutiLakshmi N Mangamoori
May 28, 2008·Clinica Chimica Acta; International Journal of Clinical Chemistry·Venkanna NarkutiLakshmi Narasu Mangamoori
Apr 13, 2007·Clinica Chimica Acta; International Journal of Clinical Chemistry·Venkanna NarkutiLakshmi N Mangamoori
Feb 11, 2015·Forensic Science International. Genetics·Yu Xuan LiuShao Hua Yi
Oct 4, 2014·Forensic Science International. Genetics·Minttu Hedman, Jukka U Palo
May 18, 2020·International Journal of Legal Medicine·Lizbeth J González-HerreraHéctor Rangel-Villalobos

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