Multistep Optimization of β-Glucosidase Extraction from Germinated Soybeans (Glycine max L. Merril) and Recovery of Isoflavone Aglycones

Foods
Luciane Yuri YoshiaraElza Iouko Ida

Abstract

Epicotyls from germinated soybeans (EGS) have great potential as sources of endogenous β-glucosidase. Furthermore, this enzyme may improve the conversion of isoflavones into their corresponding aglycones. β-Glucosidase may also increase the release of aglycones from the cell wall of the plant materials. Therefore, the aim of this work was to optimize both the extraction of β-glucosidase from EGS and to further examine its application in defatted soybean cotyledon to improve the recovery of aglycones, which were evaluated by ultra-high performance liquid chromatography (UHPLC). A multistep optimization was carried out and the effects of temperature and pH were investigated by applying a central composite design. The linear effect of pH and the quadratic effect of pH and temperature were significant for the extraction of β-glucosidase and recovery aglycones, respectively. Optimum extraction of β-glucosidase from EGS occurred at 30 °C and pH 5.0. Furthermore, the maximum recovery of aglycones (98.7%), which occurred at 35 °C and pH 7.0⁻7.6 during 144 h of germination, increased 8.5 times with respect to the lowest concentration. The higher bioaccessibility of aglycones when compared with their conjugated counterparts is well subst...Continue Reading

References

May 1, 1996·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·M FukutakeK Wakabayashi
Apr 1, 1997·Annals of Medicine·H Adlercreutz, W Mazur
Dec 16, 1998·The American Journal of Clinical Nutrition·L CowardS Barnes
May 3, 2003·The British Journal of Nutrition·Ian RowlandAedin Cassidy
Jan 7, 2004·Journal of Agricultural and Food Chemistry·Kelly R WalshMark L Failla
May 23, 2006·Applied Microbiology and Biotechnology·Lun-Cheng KuoKung-Ta Lee
Feb 22, 2007·Journal of Agricultural and Food Chemistry·Phimonphan ChuankhayanJames R Ketudat Cairns
May 13, 2008·Phytochemistry·Anne Vinther MorantSøren Bak
Jun 24, 2008·Critical Reviews in Food Science and Nutrition·Theresa LarkinLee Astheimer
Mar 10, 2009·Food Microbiology·Jose A MarazzaGraciela Savoy de Giori
Jan 6, 2011·Molecular Nutrition & Food Research·Alexander GosslauNancy E Rawson
May 25, 2012·International Journal of Food Sciences and Nutrition·Luciane Yuri YoshiaraElza Iouko Ida
Sep 26, 2013·BMC Complementary and Alternative Medicine·Carina DennyPedro L Rosalen
Feb 24, 2017·Foods·Do Tan KhangTran Dang Xuan
Mar 31, 2017·Current Pharmaceutical Design·Ryszard Amarowicz, Ronald B Pegg
Aug 3, 2017·Food Chemistry·Adriano Costa de CamargoFereidoon Shahidi
Feb 15, 2018·PloS One·Maria Augusta TremocoldiSeverino Matias de Alencar
May 10, 2018·Food Chemistry·Won Young Oh, Fereidoon Shahidi

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Citations

Dec 11, 2020·Foods·Ryszard Amarowicz
Jan 23, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Adriano Costa de CamargoAndrés R Schwember

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