Abstract
Antibody engineering has allowed for the rapid generation of binding agents against virtually any antigen of interest, predominantly for therapeutic applications. Considerably less attention has been given to the development of diagnostic reagents and biosensors using engineered antibodies. Recently, we produced a novel pentavalent bispecific antibody (i.e., decabody) by pentamerizing two single-domain antibodies (sdAbs) through the verotoxin B subunit (VTB) and found both fusion partners to be functional. Using a similar approach, we have engineered a bispecific pentameric fusion protein consisting of five sdAbs and five cellulose-binding modules (CBMs) linked via VTB. To find an optimal design format, we constructed six bispecific pentamers consisting of three different CBMs, fused to the Staphylococcus aureus-specific human sdAb HVHP428, in both orientations. One bispecific pentamer, containing an N-terminal CBM9 and C-terminal HVHP428, was soluble, non-aggregating, and did not degrade upon storage at 4 °C for over six months. This molecule was dually functional as it bound to cellulose-based filters as well as S. aureus cells. When impregnated in cellulose filters, the bispecific pentamer recognized S. aureus cells in a flo...Continue Reading
References
Dec 6, 1991·Journal of Chromatography·R L Wimalasena, G S Wilson
Oct 12, 1989·Nature·E S WardG Winter
Dec 1, 1993·Bio/technology·C RamírezD G Kilburn
Jun 3, 1993·Nature·C Hamers-CastermanR Hamers
Aug 30, 1996·The Journal of Biological Chemistry·M LinderT T Teeri
Oct 10, 1997·FEBS Letters·M Arbabi GhahroudiS Muyldermans
Apr 21, 1999·Biochimica Et Biophysica Acta·R H van der LindenC T Verrips
Mar 7, 2000·Journal of Biotechnology·L G FrenkenC T Verrips
Feb 13, 2001·Protein Engineering·B W McLeanD G Kilburn
May 4, 2001·The Journal of Biological Chemistry·J TanhaC R MacKenzie
Aug 22, 2001·The Biochemical Journal·A B BorastonD G Kilburn
Sep 22, 2001·Molecular Immunology·S D NuttallP J Hudson
Feb 16, 2002·Protein Science : a Publication of the Protein Society·Mireille DumoulinAndre Matagne
Oct 7, 2003·The Journal of Biological Chemistry·Benoit StijlemansStefan Magez
Dec 9, 2003·Journal of Molecular Biology·Jianbing ZhangC Roger MacKenzie
Jun 5, 2004·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Mojgan KavoosiCharles A Haynes
Oct 2, 2004·Biotechnology Progress·Beatriz RodriguezCharles A Haynes
Sep 10, 2005·Nature Biotechnology·Philipp Holliger, Peter J Hudson
Oct 14, 2005·The Journal of Biological Chemistry·Rebecca ToJamshid Tanha
Jan 13, 2006·Cancer Metastasis Reviews·Mehdi Arbabi-GhahroudiRoger MacKenzie
Feb 2, 2006·Applied Microbiology and Biotechnology·M M HarmsenF G van Zijderveld
May 5, 2006·Biotechnology and Bioengineering·William LewisNeil Parry
Jun 9, 2006·Microbiology and Molecular Biology Reviews : MMBR·Oded ShoseyovIlan Levy
Dec 5, 2006·Journal of Immunological Methods·Emily StoneJianbing Zhang
Jul 3, 2007·Journal of Chromatography. a·Mojgan KavoosiCharles A Haynes
Sep 12, 2007·Langmuir : the ACS Journal of Surfaces and Colloids·Gautam PanguDaniel A Hammer
Sep 25, 2007·Journal of Molecular Biology·Akiko KoideShohei Koide
Apr 15, 2008·Analytical Chemistry·Andres W MartinezGeorge M Whitesides
Oct 14, 2008·Analytical Chemistry·Weian ZhaoYingfu Li
Jun 25, 2009·Analytical Chemistry·Xu LiWei Shen
Jan 1, 2009·Sensors·Ellen R GoldmanGeorge P Anderson
Aug 8, 2008·Sensors·Dirk SaerensSerge Muyldermans
Citations
Mar 19, 2014·PloS One·Mohammad OwaisSwaleha Zubair
May 9, 2014·ACS Nano·Gustavo BodelónLuis M Liz-Marzán
Apr 11, 2014·Analytical Chemistry·Ana M M RosaD Miguel F Prazeres
Dec 25, 2012·Journal of Immunological Methods·Scott A WalperGeorge P Anderson
Nov 25, 2011·Enzyme and Microbial Technology·Karel Hernandez, Roberto Fernandez-Lafuente
Jul 30, 2014·Journal of Virological Methods·Yong Kwan KimDong-Jun An
Aug 6, 2013·Biosensors & Bioelectronics·Asta Makaraviciute, Almira Ramanaviciene
Feb 18, 2015·Biotechnology Advances·Carla OliveiraFrancisco M Gama
Mar 31, 2015·Journal of Virological Methods·Yong Kwan KimDong-Jun An
Jan 1, 2009·Sensors·Joseph Irudayaraj
Jun 16, 2017·Physical Chemistry Chemical Physics : PCCP·A M M RosaP M R Paulo
Nov 6, 2018·Chemistry : a European Journal·Valentina HilbergHarald Kolmar
Jan 11, 2011·The Journal of Biological Chemistry·Greg HussackJamshid Tanha
Feb 10, 2017·The Journal of Biological Chemistry·Virgínia M R PiresDuarte Miguel Prazeres
Apr 25, 2020·Frontiers in Microbiology·Gang ZhouZemin Fang
Dec 13, 2016·Nature Reviews. Microbiology·Lior ArtziSarah Moraïs
Nov 23, 2017·Frontiers in Immunology·Greg HussackC Roger MacKenzie
Dec 29, 2018·Scientific Reports·Lisa C Shriver-LakeEllen R Goldman
Apr 19, 2017·Journal of Molecular Recognition : JMR·A AlmeidaD M F Prazeres
Sep 6, 2017·Frontiers in Immunology·Gualberto Gonzalez-SapienzaSofía Tabares-da Rosa
Aug 14, 2014·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Julie Credou, Thomas Berthelot
Jan 28, 2021·Analytical and Bioanalytical Chemistry·Gülsen Betül AktasLluis Masip
Oct 30, 2020·Frontiers in Immunology·Sabine FlickerSergei V Tillib
Jun 8, 2017·Toxicon : Official Journal of the International Society on Toxinology·Lisa C Shriver-LakeGeorge P Anderson
Mar 30, 2017·Biotechnology Reports·Jinny L LiuGeorge P Anderson