Multivalent biomaterial platform to control the distinct arterial venous differentiation of pluripotent stem cells

Biomaterials
Taylor B DorseyGuohao Dai

Abstract

Vascular endothelial cells (ECs) differentiated from pluripotent stem cells have enormous potential to be used in a variety of therapeutic areas such as tissue engineering of vascular grafts and re-vascularization of ischemic tissues. To date, various protocols have been developed to differentiate stem cells toward vascular ECs. However, current methods are still not sufficient to drive the distinct arterial venous differentiation. Therefore, developing refined method of arterial-venous differentiation is critically needed to address this gap. Here, we developed a biomaterial platform to mimic multivalent ephrin-B2/EphB4 signaling and investigated its role in the early arterial and venous specification of pluripotent stem cells. Our results show immobilized ephrinB2 or EphB4 on hydrogel substrates have a distinct effect on arterial venous differentiation by regulating several arterial venous markers. When in combination with Wnt pathway agonist or BMP4 signaling, the ephrin-B2/EphB4 biomaterial platform can create diverging EC progenitor populations, demonstrating differential gene expression pattern across a wide range of arterial and venous markers, as well as phenotypic markers such as anti-thrombotic, pro-atherogenic and os...Continue Reading

References

Sep 5, 2002·Nature Reviews. Genetics·Nathan D Lawson, Brant M Weinstein
Dec 19, 2003·Development·Ferdinand le NobleAnne Eichmann
Dec 4, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Kimiko YamamotoJoji Ando
Jul 1, 2006·Arteriosclerosis, Thrombosis, and Vascular Biology·Takami Yurugi-KobayashiJun K Yamashita
Aug 8, 2006·Cell Metabolism·Chen ZhaoKoichi Matsuo
Dec 23, 2006·Arteriosclerosis, Thrombosis, and Vascular Biology·Fredrik LannerFilip Farnebo
Feb 13, 2007·Trends in Cardiovascular Medicine·Jun K Yamashita
Apr 28, 2007·Arteriosclerosis, Thrombosis, and Vascular Biology·Fabio A KudoAlan Dardik
Oct 31, 2008·Cell and Tissue Research·Nathaniel G dela Paz, Patricia A D'Amore
Nov 8, 2008·Journal of Applied Physiology·Syotaro ObiJoji Ando
Mar 17, 2009·Circulation Research·Matthew R Swift, Brant M Weinstein
Apr 21, 2010·The Journal of Cell Biology·Kohei YamamizuJun K Yamashita
Jul 9, 2010·Nature Medicine·Stéphane Germain, Anne Eichmann
Jul 14, 2010·Vascular Medicine·Alex Le BrasPeter Oettgen
Feb 23, 2011·The Journal of Experimental Medicine·Akihito MutoAlan Dardik
Mar 31, 2011·Stem Cells and Development·Alicia A BlancasKara E McCloskey
Jun 1, 2011·Journal of Vascular Research·Drew E GlaserKara E McCloskey
Jun 17, 2011·Arteriosclerosis, Thrombosis, and Vascular Biology·G Brandon AtkinsAnne Hamik
Jul 28, 2011·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Diana C ChongOndine Cleaver
Oct 18, 2011·Seminars in Cell & Developmental Biology·Elizabeth A V Jones
May 31, 2012·Proceedings of the National Academy of Sciences of the United States of America·Xiaojun LianSean P Palecek
Sep 18, 2012·Nature Neuroscience·Randolph S AshtonDavid V Schaffer
Sep 5, 2013·Cold Spring Harbor Perspectives in Biology·Erika M LisabethElena B Pasquale
Oct 22, 2013·Nature Nanotechnology·Anthony ConwayDavid V Schaffer
Dec 7, 2013·Stem Cells Translational Medicine·Melanie MarchandRenee A Reijo Pera

❮ Previous
Next ❯

Citations

Jul 2, 2019·Frontiers in Bioengineering and Biotechnology·Seep AroraYi-Chin Toh
Jan 11, 2019·Stem Cells International·Taylor B Bertucci, Guohao Dai

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.