Multivalent carbohydrate recognition on a glycodendrimer-functionalized flow-through chip

Chembiochem : a European Journal of Chemical Biology
Hilbert M BranderhorstRoland J Pieters


Dendrimers were fitted out with up to eight mannose moieties by "click" chemistry. They were subsequently attached to aluminum oxide chips via a spacer that was linked to the dendrimer core; this resulted in a microarray of glycodendrimers. Binding of the glycodendrimers to the fluorescent lectins ConA and GNA was observable in real time. In a single experiment it was possible to observe the multivalency enhancement or cluster effect in the binding event. This effect was small for ConA, in agreement with its widely spaced binding sites, whereas it was large for GNA, with its twelve much more closely spaced binding sites. The dendrimer-fitted chip represents a valuable screening tool for multivalency effects. Furthermore kinetic and thermodynamic data on binding events can be deduced. Inhibition experiments are also possible with the system as was shown for ConA with alpha-methyl mannose as the inhibitor.


Jan 1, 1975·International Journal of Peptide and Protein Research·G I Tesser, I C Balvert-Geers
Jun 26, 2001·Glycoconjugate Journal·R T Lee, Y C Lee
Jan 5, 2002·Organic Letters·Eric K Woller, Mary J Cloninger
Feb 14, 2002·Chemical Reviews·Milton J Kiefel, Mark von Itzstein
May 2, 2002·Chemistry & Biology·Benjamin T Houseman, Milan Mrksich
Jun 20, 2002·Journal of Biotechnology·W Bruce Turnbull, J Fraser Stoddart
Oct 31, 2002·Journal of the American Chemical Society·Zhongsheng ZhangErkang Fan
Dec 10, 2002·Current Opinion in Chemical Biology·Mary J Cloninger
Dec 12, 2002·Journal of the American Chemical Society·Jason E GestwickiLaura L Kiessling
Jan 28, 2003·Current Opinion in Chemical Biology·Heng Zhu, Michael Snyder
Jun 6, 2003·Journal of the American Chemical Society·Emily A SmithRobert M Corn
Oct 22, 2003·Current Opinion in Structural Biology·Ten FeiziChi-Huey Wong
Nov 5, 2003·Proteomics·Denong Wang
Dec 9, 2003·Chembiochem : a European Journal of Chemical Biology·Reshma AutarRoland J Pieters
Apr 15, 2004·Journal of the American Chemical Society·Sungjin ParkInjae Shin
Jun 3, 2004·Chembiochem : a European Journal of Chemical Biology·Maja KöhnJosé M García Fernández
Jul 16, 2004·Organic & Biomolecular Chemistry·Daniela ArosioAnna Bernardi
Dec 14, 2004·Journal of Medicinal Chemistry·John A F JoostenRoland J Pieters
Jan 7, 2005·Chemistry : a European Journal·Injae ShinMyung-ryul Lee
Mar 31, 2005·Chemistry : a European Journal·Daniel B Werz, Peter H Seeberger
Mar 31, 2005·Chembiochem : a European Journal of Chemical Biology·Kanoelani T PilobelloLara K Mahal
Jun 2, 2005·Journal of the American Chemical Society·Marta Gómez-GarcíaJosé M García Fernández
Sep 15, 2005·Chemical Communications : Chem Comm·Dirk T S RijkersRob M J Liskamp
Sep 22, 2005·Journal of the American Chemical Society·Kwang-Seuk KoNicola L Pohl
Sep 28, 2005·Chemistry : a European Journal·Katie J DooresBenjamin G Davis
Mar 2, 2006·Journal of the American Chemical Society·Jose L de PazPeter H Seeberger
Apr 19, 2006·Nature Chemical Biology·James C PaulsonBrian E Collins
Apr 28, 2006·Angewandte Chemie·Joseph C ManimalaJeffrey C Gildersleeve
Jul 20, 2006·Bioconjugate Chemistry·Mark L Wolfenden, Mary J Cloninger
Nov 7, 2006·Carbohydrate Research·Sergey A NepogodievRobert A Field
Dec 13, 2006·Chembiochem : a European Journal of Chemical Biology·Zhichao PeiOlof Ramström
Jan 31, 2007·Biosensors & Bioelectronics·Wolfgang VornholtMichael Keusgen
Jul 6, 2007·PloS One·Simone LemeerJeroen den Hertog
Jul 13, 2007·Chembiochem : a European Journal of Chemical Biology·Aliaksei V PukinRoland J Pieters
Aug 19, 2007·Molecular & Cellular Proteomics : MCP·Simone LemeerMonique Slijper
Dec 1, 2007·Chemical Communications : Chem Comm·Hilbert M BranderhorstRoland J Pieters


Mar 8, 2013·Carbohydrate Research·Johannes W WehnerThisbe K Lindhorst
Mar 6, 2012·Analytical Chemistry·Qi TongMingdi Yan
Dec 17, 2008·Journal of the American Chemical Society·Amanda L MartinElizabeth R Gillies
Mar 18, 2010·Chemical Communications : Chem Comm·Raghavendra KikkeriPeter H Seeberger
Jun 8, 2010·Physical Chemistry Chemical Physics : PCCP·Diego PallarolaOmar Azzaroni
Mar 17, 2012·Lab on a Chip·Satya Nandana Narla, Xue-Long Sun
Feb 13, 2013·Beilstein Journal of Organic Chemistry·Francesca PerticiRoland J Pieters
Mar 25, 2014·Glycobiology·Linlin WangLara K Mahal
May 8, 2009·Organic & Biomolecular Chemistry·Roland J Pieters
May 8, 2010·Organic & Biomolecular Chemistry·Núria Parera PeraRoland J Pieters
Jan 29, 2011·Chemical Science·Song-Gil LeeLinda C Hsieh-Wilson
Mar 20, 2012·Chemical Communications : Chem Comm·Francesca Pertici, Roland J Pieters
Apr 19, 2013·Chemical Society Reviews·Laura L Kiessling, Joseph C Grim
Nov 26, 2009·Expert Review of Proteomics·Chi-Hui Liang, Chung-Yi Wu
Feb 4, 2014·Current Opinion in Chemical Biology·Xuezheng SongDavid F Smith
Feb 22, 2012·Biosensors & Bioelectronics·Oscar NorbergOlof Ramström
Dec 10, 2015·Organic & Biomolecular Chemistry·Madanodaya SundhoroMingdi Yan
Dec 26, 2015·Chemical Reviews·Martina DelbiancoPeter H Seeberger
Jan 1, 2009·Drug Discovery Today. Technologies
Sep 13, 2012·Journal of the American Chemical Society·Kamil Godula, Carolyn R Bertozzi
Jul 25, 2009·Current Opinion in Chemical Biology·Oyindasola Oyelaran, Jeffrey C Gildersleeve
May 23, 2012·Chemistry, an Asian Journal·Xizhe TianInjae Shin
Jul 31, 2010·Chembiochem : a European Journal of Chemical Biology·Núria Parera PeraRoland J Pieters
May 7, 2014·Chemistry, an Asian Journal·Chen-Wei LiKwok-Kong Tony Mong
May 11, 2015·Glycoconjugate Journal·Satya Nandana NarlaXue-Long Sun
Apr 15, 2016·Biomaterials Science·P Bojarová, V Křen
Nov 15, 2012·Analytical Biochemistry·Hilde van HattumRoland J Pieters
Nov 30, 2012·Chemical Society Reviews·Sungjin ParkInjae Shin
Oct 4, 2014·The Analyst·María J MarínDavid A Russell
Jun 13, 2017·Organic & Biomolecular Chemistry·Antoine HoangOlivier Renaudet
Oct 9, 2012·Macromolecular Bioscience·Rajesh Sunasee, Ravin Narain
Jul 13, 2019·Faraday Discussions·Marco MendePeter H Seeberger
Jul 25, 2019·Faraday Discussions·J Sebastian TemmeJeffrey C Gildersleeve
Apr 22, 2020·Chemistry : a European Journal·Marco MendeFelix F Loeffler
May 18, 2016·Experimental Biology and Medicine·Mia L HuangKamil Godula

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