Multivalent Glycosylation of Fluorescent Gold Nanoclusters Promotes Increased Human Dendritic Cell Targeting via Multiple Endocytic Pathways

ACS Applied Materials & Interfaces
Xavier Le GuévelCristobalina Mayorga

Abstract

We report the synthesis and characterization of gold nanoclusters (Au NCs) stabilized by a mixture of zwitterionic and multivalent mannose ligands. Characterization of this carbohydrated nanosystem confirms its small size (∼2 nm), intense red-NIR fluorescence, relatively high affinity to lectin (ConA), and stability in physiological media. Cell studies performed using human-monocyte-derived dendritic cells (DCs) show that Au NC uptake efficiency is greatly enhanced by the presence of surface carbohydrate (>250% compared to noncarbohydrated Au NCs), allowing their detection in cells by fluorescence following incubation with concentrations as low as 1 μg mL(-1). Investigation using electron microscopy and pharmacological inhibitors indicates that Au NC uptake is mediated by multiple endocytic pathways involving the engulfment of Au NCs into endosomes and partial transport to lysosomes. Results show that clathrin- and F-actin-dependent pathways play major roles in Au NC uptake by DCs, regardless of whether or not they are coated with carbohydrates. In contrast, a specific C-lectin inhibitor induces a 60% decrease in DC particle uptake only for the carbohydrate-coated Au NCs. This study demonstrates that the combination of ultrasma...Continue Reading

References

Mar 26, 2002·Nature Reviews. Immunology·Carl G FigdorGosse J Adema
Feb 18, 2005·Nature Immunology·Abul K Abbas, Arlene H Sharpe
Mar 2, 2006·Pharmacological Reviews·Ikramy A KhalilHideyoshi Harashima
Mar 15, 2006·Journal of Controlled Release : Official Journal of the Controlled Release Society·Sai T ReddyMelody A Swartz
May 17, 2006·Immunology and Allergy Clinics of North America·Natalija Novak
Jun 3, 2006·Developmental Cell·Eric MaciaTomas Kirchhausen
Nov 2, 2006·Journal of Cell Science·Sestina FalconeJacopo Meldolesi
Nov 16, 2006·Annual Review of Physical Chemistry·Jie ZhengRobert M Dickson
Sep 18, 2007·Nature Biotechnology·Sai T ReddyJeffrey A Hubbell
Apr 15, 2008·Journal of the American Chemical Society·Manzhou ZhuRongchao Jin
Jul 26, 2008·Nature Nanotechnology·Marina A Dobrovolskaia, Scott E McNeil
Apr 1, 2009·Journal of Controlled Release : Official Journal of the Controlled Release Society·Hae Yun NamSeo Young Jeong
May 23, 2009·Journal of Cell Science·Chun-Lin ChenJung San Huang
Nov 27, 2009·Nature·Jeffrey A HubbellMelody A Swartz
Jan 21, 2010·Nanomedicine : Nanotechnology, Biology, and Medicine·Rebecca Klippstein, David Pozo
Feb 17, 2010·Journal of Controlled Release : Official Journal of the Controlled Release Society·Luis J CruzCarl G Figdor
May 5, 2010·The Journal of Physical Chemistry. C, Nanomaterials and Interfaces·Chen ZhouJie Zheng
Jun 17, 2010·Nano Letters·Zhikun Wu, Rongchao Jin
Jul 21, 2010·Nanoscale·Rongchao Jin
Jan 7, 2011·American Journal of Physiology. Cell Physiology·Kenneth W DunnJohn H McDonald
Mar 5, 2011·Angewandte Chemie·Chen ZhouJie Zheng
Jul 29, 2011·Nanomedicine·Leshuai W ZhangNancy A Monteiro-Riviere
Jul 30, 2011·Chemical Society Reviews·Yuning HongBen Zhong Tang
Jan 5, 2012·ACS Nano·María MorosJesús M de la Fuente
Mar 1, 2012·Journal of Controlled Release : Official Journal of the Controlled Release Society·Wendy W J UngerYvette van Kooyk
Jun 19, 2012·Nanoscale·Jie ZhengJinbin Liu
Oct 18, 2012·Cancer Research·Shuaidong HuoXing-Jie Liang
Oct 30, 2012·Molecular Immunology·Juan J García-VallejoYvette van Kooyk
Dec 21, 2012·Chemical Society Reviews·Anna BernardiAnne Imberty
Jan 4, 2013·Biomacromolecules·Joanna LuczkowiakJavier Rojo
Jan 5, 2013·Chemical Society Reviews·Marco MarradiSoledad Penadés
Feb 8, 2013·Blood·Martin KreutzCarl G Figdor

❮ Previous
Next ❯

Citations

Jan 20, 2016·Expert Review of Vaccines·Joshua J GlassRobert De Rose
Jun 4, 2016·Current Opinion in Allergy and Clinical Immunology·Cristobalina MayorgaMaria I Montañez
Sep 14, 2016·ACS Applied Materials & Interfaces·Wenshan LiCongcong Xu
Sep 27, 2018·Chemistry, an Asian Journal·Jing HuJian Yin
Mar 7, 2019·Chemical Society Reviews·Xi Kang, Manzhou Zhu
Jul 8, 2017·Beilstein Journal of Organic Chemistry·Federica CompostellaLaura Polito
Jan 10, 2020·Chemical Society Reviews·Baptiste ThomasSébastien Vidal
Dec 14, 2017·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Puneet Khandelwal, Pankaj Poddar
Apr 28, 2018·International Journal of Molecular Sciences·Elisa PanzariniLuciana Dini
Dec 29, 2020·Biomaterials Science·Ying LinXiqun Jiang
Feb 10, 2021·Allergy·Cristobalina MayorgaMaria Isabel Montañez
May 13, 2021·Chemical Communications : Chem Comm·Javier Ramos-Soriano, Javier Rojo
May 29, 2021·Frontiers in Chemistry·Dongyoon KimChristoph Rademacher
Nov 16, 2017·ACS Applied Materials & Interfaces·Wilke C de VriesBart Jan Ravoo
Aug 23, 2021·Current Opinion in Allergy and Clinical Immunology·Alessandra LongoPaolo Colombo

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.