Multivalent ion-based in situ gelling polysaccharide hydrogel as an injectable bone graft

Carbohydrate Polymers
Sun Woo JungJin Ho Lee

Abstract

We prepared in situ gelling alginate (ALG)/hyaluronic acid (HA) hydrogels with a controllable gelation rate using CaSO4 as a crosslinking agent and Na2HPO4 as a crosslinking retardation agent. The ALG/HA hydrogels provided sustained release of bone morphogenetic protein-2 (BMP-2) immobilized in the hydrogels over 5 weeks. The BMP-2-immobilized ALG/HA hydrogels with different ALG/HA ratios were investigated for their in vitro osteogenic differentiation behavior of human bone marrow stem cells (hBMSCs) and in vivo bone regeneration behavior using an animal model (mandibular defect model of miniature pigs). Our findings from cell culture and animal study demonstrated that the osteogenic differentiation of hBMSCs was improved with increasing HA composition in the hydrogel. The hBMSCs/BMP-2-immobilized ALG/HA hydrogel allowed greatly enhanced osteogenic differentiation of hBMSCs (in vitro) and bone regeneration (in vivo) compared with the ALG/HA hydrogel itself and single hBMSCs- or BMP-2-immobilized hydrogel groups.

Citations

Nov 28, 2019·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Chen LiuWei Zheng
Nov 16, 2019·Angewandte Chemie·Margarita Vázquez-González, Itamar Willner
Mar 15, 2018·International Journal of Molecular Sciences·Kristina GlenskeMike Barbeck
Jan 25, 2019·Gels·Víctor H Pérez-Luna, Orfil González-Reynoso
Oct 19, 2019·Tissue Engineering and Regenerative Medicine·Sun Woo JungJin Ho Lee
Jan 19, 2021·ACS Biomaterials Science & Engineering·Hee Yun JangJin Ho Lee
Oct 20, 2020·Journal of Oral Biology and Craniofacial Research·Divya MehrotraR K Singh
Mar 11, 2019·ACS Biomaterials Science & Engineering·In Gul KimJin Ho Lee
Feb 9, 2021·Chemistry of Materials : a Publication of the American Chemical Society·Soyon Kim, Min Lee

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