Multiview microscopy of single cells through microstructure-based indirect optical manipulation

Biomedical Optics Express
Gaszton VizsnyiczaiLóránd Kelemen

Abstract

Fluorescent observation of cells generally suffers from the limited axial resolution due to the elongated point spread function of the microscope optics. Consequently, three-dimensional imaging results in axial resolution that is several times worse than the transversal. The optical solutions to this problem usually require complicated optics and extreme spatial stability. A straightforward way to eliminate anisotropic resolution is to fuse images recorded from multiple viewing directions achieved mostly by the mechanical rotation of the entire sample. In the presented approach, multiview imaging of single cells is implemented by rotating them around an axis perpendicular to the optical axis by means of holographic optical tweezers. For this, the cells are indirectly trapped and manipulated with special microtools made with two-photon polymerization. The cell is firmly attached to the microtool and is precisely manipulated with 6 degrees of freedom. The total control over the cells' position allows for its multiview fluorescence imaging from arbitrarily selected directions. The image stacks obtained this way are combined into one 3D image array with a multiview image processing pipeline resulting in isotropic optical resolution...Continue Reading

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Citations

Sep 26, 2020·Micromachines·István GrexaLóránd Kelemen
Feb 9, 2021·Frontiers in Bioengineering and Biotechnology·Pisrut PhummiratDaryl Preece
Apr 9, 2021·Scientific Reports·Stefano FerrettiRoberto Di Leonardo
Jul 6, 2021·Biomedical Optics Express·Jiawei SunJürgen W Czarske

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