Mummy Prevents IL-1β-Induced Inflammatory Responses and Cartilage Matrix Degradation via Inhibition of NF-қB Subunits Gene Expression in Pellet Culture System

Advanced Pharmaceutical Bulletin
Fereshteh MorrovatiAzadeh Montaseri

Abstract

Purpose: In Persian traditional medicine, application of Mummy material has been advised since hundred years ago for treatment of different diseases as bone fracture, cutaneous wounds and joint inflammation. Regarding to the claim of indigenous people for application of this material in the treatment of joint inflammation, the present study was designed to evaluate whether Mummy can revoke the inflammatory responses in chondrocytes stimulated with interleukin 1-β (IL-1β). Methods: Isolated chondrocytes at the second passage were plated in 50 ml conical tubes at density of 1x106 for pellet culture or were plated in T75 culture flasks as monolayer. Cells in both groups were treated as control (receiving serum free culture medium), negative control (receiving IL-1β (10ng/ml for 24 hr)) and IL-1β pre-stimulated cells which treated with Mummy at concentrations of 500 and 1000µg/ml for 72hrs. After 72 hrs, to evaluate whether Mummy can revoke the inflammatory response in chondrocytes, cell in different groups were prepared for investigation of gene expression profile of collagen II, Cox-2, MMP-13, C-Rel and P65 using real-time RT-PCR. Results: Treatment of chondrocytes with IL-1β (10ng/ml) resulted in a significant increase in e...Continue Reading

References

Apr 26, 2003·Matrix Biology : Journal of the International Society for Matrix Biology·Ranieri CanceddaRodolfo Quarto
Jan 9, 2007·Ultrasound in Medicine & Biology·Kwideok ParkKaren Hasty
Nov 3, 2007·Advanced Drug Delivery Reviews·Cindy Chung, Jason A Burdick
Jul 27, 2011·Current Opinion in Rheumatology·Mary B Goldring, Miguel Otero
Jul 17, 2012·Osteoarthritis and Cartilage·M M J CaronT J M Welting
Jan 16, 2016·Evidence-based Complementary and Alternative Medicine : ECAM·Yudi ZhangShike Shang

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Citations

Jan 7, 2021·Tissue Engineering. Part C, Methods·Meagan J MakarczykHang Lin
Nov 17, 2020·Dose-response : a Publication of International Hormesis Society·Daqian WanLiming Cheng

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