PMID: 4329967Aug 1, 1971

Mumps virus replication in chick embryo lung cells: properties of ribonucleic acids in virions and infected cells

Journal of Virology
J L East, D W Kingsbury

Abstract

Ribonucleic acid (RNA) species in mumps virions and in infected cells were compared. The predominant RNA species in virions labeled with (3)H-uridine sedimented at 50S; RNA species sedimenting at 28, 18, and about 10S were also present. The virion-associated RNA species sedimenting slower than 50S contained some nucleotide sequences similar to 50S virion RNA. Although mumps virus replication was severely inhibited by high concentrations of actinomycin D, some virus was made, and virus-specific RNA species accumulated in infected cells. Mumps virus resembled other paramyxoviruses in inducing, in infected cells, synthesis not only of 50S RNA but also of slower sedimenting RNA species with a peak distribution at about 18S, complementary in base sequences to 50S virion RNA. In addition, base sequences of the parental type were relatively abundant in the RNA species sedimenting slower than 50S; these may represent precursors of the slowly sedimenting RNA species associated with virions. Ribonuclease-resistant RNA was detected in infected cells; this may represent replicative or transcriptive intermediates. Inhibition of protein synthesis with cycloheximide severely depressed accumulation of labeled 50S RNA in infected cells but did ...Continue Reading

Citations

Jan 1, 1982·Microbiology and Immunology·T Matsumoto
Jan 1, 1986·Medical Microbiology and Immunology·H NaruseY Nagai
Jan 1, 1974·Medical Microbiology and Immunology·D W Kingsbury
Dec 27, 1977·Medical Microbiology and Immunology·H I HuppertzV ter Meulen

Related Concepts

Centrifugation, Density Gradient
Centrifugation, Zonal
Chick Embryo
Actidione
Cytopathogenic Effect, Viral
Ac-De
Fibroblasts
Lung
Mumps virus
RNA Denaturation

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