PMID: 3754306Apr 1, 1986Paper

Murine bone marrow culture system for cytogenetic analysis

Mutation Research
G KrishnaT Ong

Abstract

A mouse bone marrow culture system for examining genotoxicity of agents by first exposing animals in vivo then growing cells in vitro is presented. This assay can also be used for in vitro and/or for the in vivo and in vitro comparative cytogenetic studies. The protocol involves culturing of approximately 1,000,000 nucleated cells obtained from mice tibia and femora in 5 ml of Ham's F-12 medium containing 20% fetal bovine serum, 10% whole uterus extract from pregnant mice and 1% penicillin-streptomycin. The use of flasks and mouse uterus extract for culturing are important steps for higher mitotic yield. The addition of 20 microM BrdU for 24 h helps in the differentiation of sister chromatids for sister-chromatid exchange (SCE) analysis. Cyclophosphamide, given to mice through intraperitoneal injection, induced significant dose-related SCEs in culture. Trinitrofluorenone, a direct-acting mutagen, caused dose-related SCEs in in vitro bone marrow cell culture.

References

Apr 1, 1976·Experimental Cell Research·A P Craig-Holmes, M W Shaw
May 1, 1979·Mutation Research·Y Nakanishi, E L Schneider
Feb 9, 1978·Nature·A V CarranoJ L Minkler
Jul 1, 1976·Experimental Cell Research·E L SchneiderR R Tice
Oct 1, 1985·Mutation Research·G KrishnaT Ong
Aug 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·S A Latt
Jun 1, 1966·The Australian Journal of Experimental Biology and Medical Science·T R Bradley, D Metcalf
Dec 1, 1971·The Australian Journal of Experimental Biology and Medical Science·T R BradleyM A Sumner
Sep 1, 1984·Cancer Genetics and Cytogenetics·M D Levin, O M Garson
Jan 1, 1980·Environmental Mutagenesis·G T Roberts, J W Allen

❮ Previous
Next ❯

Citations

Jan 1, 1992·International Archives of Occupational and Environmental Health·J KarelováL Hano
Sep 1, 1993·Mutation Research·J D TuckerA V Carrano
Feb 19, 2009·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Karim IskanderAnil K Jaiswal
Mar 13, 2010·Journal of Orthopaedic Surgery and Research·Hoyan LamYi-Xian Qin
Jan 1, 1988·Environmental and Molecular Mutagenesis·W Hadnagy, N H Seemayer

❮ Previous
Next ❯

Related Concepts

Related Feeds

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.