Murine complement deficiency ameliorates acute cigarette smoke-induced nasal damage.

Otolaryngology--head and Neck Surgery : Official Journal of American Academy of Otolaryngology-Head and Neck Surgery
Kara S DavisCarl Atkinson

Abstract

Exposure to cigarette smoke is a risk factor for chronic rhinosinusitis. Current literature confirms complement fragments are activated in human nasal mucosa. The mechanism(s) responsible for this activation is unclear. We investigated the effects of cigarette smoke on nasal mucosa in vitro and via a model of cigarette smoke exposure by using animals deficient in complement components. Prospective, controlled animal and in vitro human cell line study. University laboratory. Human respiratory epithelial cells were exposed to five, 10, and 20 percent cigarette smoke extract (CSE) in vitro in the presence or absence of human serum. Complement activation was assessed by enzyme-linked immunosorbent assay and immunofluorescent techniques. Complement-deficient (C3(-/-), n = 6; factor B(-/-), n = 50) and sufficient mice (wild type, n = 10) were exposed to the smoke of four cigarettes per exposure for two exposures per day for three days. Mice were sacrificed 12 hours after the last exposure, and the nasal cavity was surgically removed. Histological characteristics were analyzed by the use of a subjective scale and quantitative image analysis scoring systems. In vitro analysis of respiratory cell cultures demonstrated that exposure of s...Continue Reading

References

Apr 26, 2003·American Journal of Respiratory Cell and Molecular Biology·Anthony A FloreaniArt J Heires
Dec 4, 2004·Otolaryngology--head and Neck Surgery : Official Journal of American Academy of Otolaryngology-Head and Neck Surgery·Eli O MeltzerUNKNOWN American Rhinologic Society
Dec 22, 2004·Archives of Otolaryngology--head & Neck Surgery·Joseph VandermeerRobert P Schleimer
May 2, 2006·Otolaryngology--head and Neck Surgery : Official Journal of American Academy of Otolaryngology-Head and Neck Surgery·Robin LindsayWilliam E Bolger
Jan 8, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Emilie DozIsabelle Couillin
Sep 2, 2008·The Laryngoscope·Steven M Houser, Kevin J Keen
Sep 13, 2008·American Journal of Rhinology·Robert C KernRobert P Schleimer
Nov 26, 2008·The Laryngoscope·Jamie R LitvackTimothy L Smith
Jun 30, 2009·The Journal of Allergy and Clinical Immunology·David D TieuRobert P Schleimer
Aug 18, 2009·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·T KawaiE Noguchi
Jan 30, 2010·American Journal of Rhinology & Allergy·Rodney J SchlosserCarl Atkinson

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Citations

Mar 3, 2011·Current Infectious Disease Reports·Stella Lee, Andrew P Lane
Apr 11, 2012·Current Opinion in Otolaryngology & Head and Neck Surgery·Thomas S Higgins, Douglas D Reh
Apr 5, 2014·The Journal of Allergy and Clinical Immunology·Jennifer K MulliganRodney J Schlosser
Feb 21, 2018·Otolaryngology--head and Neck Surgery : Official Journal of American Academy of Otolaryngology-Head and Neck Surgery·Dallin N ChristensenEugene H Chang
Aug 29, 2018·Clinical Otolaryngology : Official Journal of ENT-UK ; Official Journal of Netherlands Society for Oto-Rhino-Laryngology & Cervico-Facial Surgery·Guilherme Pilla CaminhaMarcia Margaret Menezes Pizzichini
Jun 17, 2018·Mucosal Immunology·Jennifer K MulliganCarl Atkinson

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Methods Mentioned

BETA
enzyme-linked immunosorbent assay
ELISAs
ELISA

Software Mentioned

Prism
SigmaStat
Sample Power

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