Murine Cyp1a-1 induction in mouse hepatoma Hepa-1C1C7 cells by myristicin

Biochemical and Biophysical Research Communications
H G JeongK H Yang

Abstract

Mouse hepatoma Hepa-1c1c7 (Hepa-1) cells were treated with myristicin to assess the role of myristicin in the process of Cyp1a-1 induction. Treatment of Hepa-1 cells with myristicin increased Cyp1a-1 transcription in a dose-dependent manner as shown by analysis of 7-ethoxyresorufin O-deethylase activity, Cyp1a-1 protein level, and Cyp1a-1 mRNA. Myristicin, however, did not competitively displace [3H]2,3,7,8-tetrachlorodibenzo-p-dioxin from the Hepa-1 cytosolic aryl hydrocarbon (Ah) receptor in a competitive Ah receptor binding analysis using sucrose density gradient sedimentation and did not affect formation of DNA-protein complexes between the Ah receptor and its DRE target in a gel mobility shift assay using oligonucleotides corresponding to DRE 3 of the Cyp1a-1. These results suggest that the induction of Cyp1a-1 gene expression by myristicin in Hepa-1 cells might occur through an Ah receptor-independent pathway.

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Citations

Jul 27, 2007·Journal of Toxicology and Environmental Health. Part a·Eun Hee HanHye Gwang Jeong
Jan 24, 2003·Annual Review of Pharmacology and Toxicology·Michael S Denison, Scott R Nagy
Apr 8, 2018·Mucosal Immunology·Bruno LamasHarry Sokol
Oct 6, 2004·Archives of Pharmacal Research·Ji Young KimHye Gwang Jeong
Nov 4, 2000·Biochemical and Biophysical Research Communications·H G JeongC Y Choi

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