Murine cytomegalovirus infection-induced polyclonal B cell activation is independent of CD4+ T cells and CD40

Virology
G KarupiahH C Morse

Abstract

The results of this study demonstrate that murine cytomegalovirus (MCMV) induces polyclonal B cell activation in mice during the acute phase of primary infection. First flow cytometric analysis revealed that surface expression of CD45R, IgM, and IgK by splenocytes from MCMV-infected mice was significantly reduced with a concomitant increase in the frequency of surface IgG-expressing cells. Second, ELIspot assays demonstrated that the changes revealed by flow cytometry were paralleled by increases in the numbers of IgG-producing cells, especially those secreting IgG2a. Third, the IgG antibodies from MCMV-infected animals reacted against a variety of self and foreign antigens. MCMV-induced B cell activation was independent of CD4+ T-cell-mediated help and CD40, since activation was observed in two models of mice deficient for this T cell subset and in mice deficient for CD40. Reverse transcription-polymerase chain reaction analysis showed that mRNA transcripts for the cytokines IL-6, IL-10, and IFN-gamma were rapidly induced following infection with MCMV, but only IL-6 and IFN-gamma proteins were detectable by ELISA. In addition, the numbers of cells producing IL-6 and IFN-gamma were significantly increased in the spleen. The mag...Continue Reading

References

Apr 1, 1992·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·T A Banks, B T Rouse
Jan 1, 1992·International Reviews of Immunology·J C BowenB T Rouse
Feb 1, 1991·Research in Immunology·M FialaH V Vinters
Mar 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·N OyaizuS Pahwa
Apr 1, 1989·The Journal of Experimental Medicine·S JonjićU H Koszinowski
Jan 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·T HiranoF Sakiyama
Jun 1, 1987·The Journal of Experimental Medicine·D M Klinman, A D Steinberg
Feb 1, 1981·The Journal of Infectious Diseases·J D ShanleyJ G Stevens
Dec 1, 1983·The Journal of Experimental Medicine·L M Hutt-FletcherM H Elkins
Sep 1, 1994·Immunology Today·F H DurieR J Noelle
Jul 1, 1993·Journal of Virology·G KarupiahR M Buller
Jan 1, 1993·Virchows Archiv. B, Cell Pathology Including Molecular Pathology·C A Bruggeman
Jan 21, 1997·Proceedings of the National Academy of Sciences of the United States of America·L P CousensC A Biron

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Citations

Oct 10, 2007·Immunology and Cell Biology·Vijay PanchanathanGunasegaran Karupiah
Aug 30, 2001·International Immunology·D Markine-GoriaynoffJ P Coutelier
Oct 13, 2006·Journal of Virology·Elizabeth M Sitati, Michael S Diamond
Jun 15, 2004·Journal of Virology·Sarah E BluttMargaret E Conner
Jan 5, 2012·Toxicologic Pathology·Jerrold M WardHerbert C Morse
Oct 6, 2007·Immunologic Research·J E Butler, Marek Sinkora
Mar 6, 2007·Cytokine & Growth Factor Reviews·Eva V Acosta-RodríguezAdriana Gruppi
Feb 17, 2007·Journal of Virological Methods·Alice A BickerstaffCharles H Cook
Dec 23, 2004·Journal of Infection and Chemotherapy : Official Journal of the Japan Society of Chemotherapy·Hiroto KanekoMasafumi Taniwaki
Feb 2, 2000·International Immunology·D Markine-GoriaynoffJ P Coutelier
Jul 7, 2007·Journal of Leukocyte Biology·Carolina L MontesAdriana Gruppi
Feb 24, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·S NodaY Koga
Jun 4, 2019·Frontiers in Immunology·John E ButlerXuehui Cai
Feb 5, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·M Y SangsterP C Doherty
Dec 21, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·A KumanogohH Kikutani

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