Murine cytomegalovirus major immediate-early protein 3 interacts with cellular and viral proteins in viral DNA replication compartments and is important for early gene activation.

The Journal of General Virology
Francisco Puerta MartínezQiyi Tang

Abstract

Murine cytomegalovirus (MCMV) immediate-early protein 3 (IE3) is essential for successful viral infection. This study developed MCMVs with an EGFP-fused IE3 gene in order to study IE3 gene expression, subnuclear distribution and biological function, as well as to examine the interaction of IE3 with cellular and viral proteins. The generated viruses included MCMVIE3gfp, in which IE1 was completely removed by the in-frame fusion of exons 3 and 5 and the C terminus of IE3 was tagged with EGFP, and MCMVIE1/3gfp, in which IE1 was kept intact and EGFP was also fused to the C terminus of IE3. Unlike human CMV (HCMV), whose growth was significantly reduced when IE2 (the HCMV homologue of IE3 in MCMV) was tagged with EGFP, MCMVs with IE3-EGFP presented an unchanged replication profile. Using these new constructs, the distribution of IE3 was revealed as well as its interaction with viral and cellular proteins, especially proteins pertaining to DNA replication (M44 and E1) and cellular intrinsic defence [promyelocytic leukemia protein and histone deacetylases (HDACs)]. It was also shown that IE3 domains co-localize with DNA replication domains, and IE3 attracted other required proteins into IE3 domains via protein-protein interactions. In...Continue Reading

References

Dec 1, 1985·Journal of Virology·R M Stenberg, M F Stinski
Nov 1, 1984·Journal of Clinical Microbiology·Y EizuruY Minamishima
Dec 1, 1996·The Journal of General Virology·M MendelsonJ Sinclair
Jan 1, 1996·Intervirology·R M Stenberg
Oct 6, 1997·Journal of Virology·D M LukacJ C Alwine
Oct 9, 1999·Journal of Virology·L Wiebusch, C Hagemeier
Mar 9, 2000·Trends in Microbiology·E A FortunatoD H Spector
Dec 2, 2004·Proceedings of the National Academy of Sciences of the United States of America·Michael NevelsThomas Shenk
Feb 26, 2005·Nucleic Acids Research·Søren WarmingNeal G Copeland
Dec 22, 2007·Medical Microbiology and Immunology·Mark F Stinski, Hiroki Isomura
Jul 22, 2008·Current Topics in Microbiology and Immunology·M F Stinski, D T Petrik
Jul 22, 2008·Current Topics in Microbiology and Immunology·M J ReddehaseN K A Grzimek

❮ Previous
Next ❯

Citations

Jun 15, 2011·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Hui WangFeng Fang
May 24, 2013·Viruses·Xue-feng LiuMary Hummel
Sep 13, 2013·Journal of Virology·Qiyi TangYasuhiro Yamamura
Jul 19, 2017·The EMBO Journal·Jonathan MaelfaitJan Rehwinkel

❮ Previous
Next ❯

Methods Mentioned

BETA
transfection
PCR
co-immunoprecipitation
pull down
co-IP
FCS
FACS

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.